Cervical cancer (CC) is the most common gynecological malignancy, with high incidence and mortality rates in China. The microRNA miR‐485‐5p has previously been reported to serve as a negative regulator of tumorigenesis in breast cancer and hepatocellular carcinoma, and miR‐485‐5p has been observed to be differentially expressed between CC and normal control tissue. Here, we confirmed that miR‐485‐5p expression is lower in CC than in adjacent normal tissue and proceeded to investigate the effects of miR‐485 on tumor behavior in CC cell lines. We report that miR‐485‐5p transcription is decreased in HPV‐infected CC tissue, and levels of miR‐485 in clinical samples are positively correlated with the 5‐year overall survival rate. The Transwell assay showed that miR‐485‐5p inhibited cell invasion and migration but had no influence on apoptosis and cell proliferation. Using a luciferase reporter assay, we demonstrated that miR‐485‐5p partially abrogated cell migration and proliferation by targeting FLOT‐1 mRNA. Transfection of HPV‐infected cervical carcinoma cells with an adenovirus vector encoding human FLOT‐1 partially diminished the inhibitory effects of miR‐485 on cell invasion. Taken, together, these data demonstrated that miR‐485‐5p suppresses the invasion of cancer cells by targeting FLOT‐1 in HPV‐infected cervical carcinoma cells.

中文翻译：

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HPV感染的宫颈癌中miR-485-5p水平降低促进细胞增殖并增强侵袭能力。

宫颈癌（cervical cancer，CC）是最常见的妇科恶性肿瘤，在我国发病率和死亡率都很高。microRNA miR-485-5p 先前已被报道作为乳腺癌和肝细胞癌肿瘤发生的负调节剂，并且观察到 miR-485-5p 在 CC 和正常对照组织之间差异表达。在这里，我们证实 miR-485-5p 在 CC 中的表达低于邻近的正常组织，并着手研究 miR-485 对 CC 细胞系中肿瘤行为的影响。我们报告说，在 HPV 感染的 CC 组织中 miR-485-5p 转录减少，临床样本中 miR-485 的水平与 5 年总生存率呈正相关。Transwell实验显示miR-485-5p抑制细胞侵袭和迁移，但对细胞凋亡和增殖没有影响。使用荧光素酶报告基因测定，我们证明了 miR-485-5p 通过靶向 FLOT-1 mRNA 部分消除了细胞迁移和增殖。用编码人 FLOT-1 的腺病毒载体转染 HPV 感染的宫颈癌细胞，部分减弱了 miR-485 对细胞侵袭的抑制作用。总之，这些数据表明 miR-485-5p 通过靶向 HPV 感染的宫颈癌细胞中的 FLOT-1 来抑制癌细胞的侵袭。用编码人 FLOT-1 的腺病毒载体转染 HPV 感染的宫颈癌细胞，部分减弱了 miR-485 对细胞侵袭的抑制作用。总之，这些数据表明 miR-485-5p 通过靶向 HPV 感染的宫颈癌细胞中的 FLOT-1 来抑制癌细胞的侵袭。用编码人 FLOT-1 的腺病毒载体转染 HPV 感染的宫颈癌细胞，部分减弱了 miR-485 对细胞侵袭的抑制作用。总之，这些数据表明 miR-485-5p 通过靶向 HPV 感染的宫颈癌细胞中的 FLOT-1 来抑制癌细胞的侵袭。