Xylanase is a member of an important family of enzymes that has been used in many biotechnological processes. However, the overall cost of enzyme production has been the main problem in the industrial application of enzymes. To obtain maximum xylanase production, statistical approaches based on the Plackett–Burman design and response surface methodology were employed. The results of the statistical analyses demonstrated that the optimal conditions for increased xylanase production were the following: inoculum size, 3.8%; maize meal, 4.5%; histidine, 0.6%; methanol, 1%; culture volume, 20%; bean pulp, 30 g L−1; and Tween‐80, 0.8%; and pH 5.0. Verification of the optimization demonstrated that 3273 U mL−1 xylanase was observed under the optimal conditions in shake flask experiments. SDS–PAGE results showed that the size of xylanase protein was about 23 kDa. The results showed that the xylanase produced by fermentation came from Aspergillus Niger by MALDI‐TOF‐MS. The optimized medium resulted in 2.1‐ and 1.4‐fold higher the activity of xylanase compared with the unoptimized medium (the main nutrients are maize meal and bean pulp) and laboratory medium (the main nutrients are yeast extract and peptone), respectively. The optimization of fermentation conditions is an effective means to reduce production cost and improve xylanase activity.

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毕赤酵母GS115生产重组木聚糖酶发酵条件的优化

木聚糖酶是一个重要的酶家族的成员，已被用于许多生物技术过程。然而，酶生产的总体成本一直是酶工业应用的主要问题。为了获得最大的木聚糖酶产量，采用了基于 Plackett-Burman 设计和响应面方法的统计方法。统计分析结果表明，提高木聚糖酶产量的最佳条件如下：接种量，3.8%；玉米粉，4.5%；组氨酸，0.6%；甲醇，1%；培养量，20%；豆浆，30 g L−1；和吐温 80，0.8%；和 pH 值 5.0。优化验证表明，在摇瓶实验的最佳条件下观察到 3273 U mL-1 木聚糖酶。SDS-PAGE 结果表明木聚糖酶蛋白的大小约为 23 kDa。MALDI-TOF-MS结果表明发酵产生的木聚糖酶来自黑曲霉。与未优化的培养基（主要营养成分是玉米粉和豆浆）和实验室培养基（主要营养成分是酵母提取物和蛋白胨）相比，优化后的培养基分别使木聚糖酶的活性提高了 2.1 倍和 1.4 倍。优化发酵条件是降低生产成本、提高木聚糖酶活性的有效手段。与未优化的培养基（主要营养成分是玉米粉和豆浆）和实验室培养基（主要营养成分是酵母提取物和蛋白胨）相比，木聚糖酶的活性分别提高了 4 倍。优化发酵条件是降低生产成本、提高木聚糖酶活性的有效手段。与未优化的培养基（主要营养成分是玉米粉和豆浆）和实验室培养基（主要营养成分是酵母提取物和蛋白胨）相比，木聚糖酶的活性分别提高了 4 倍。优化发酵条件是降低生产成本、提高木聚糖酶活性的有效手段。