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Development and characterization of a fiber optical fluorescence sensor for the online monitoring of biofilms and their microenvironment
Engineering in Life Sciences ( IF 2.7 ) Pub Date : 2020-03-02 , DOI: 10.1002/elsc.201900140
Jana Schlaugat 1 , Kai Patzer 1 , Thorleif Hentrop 1 , Dörte Solle 1 , Iliyana Pepelanova 1 , Uwe Schröder 2 , Thomas Scheper 1
Affiliation  

The growth of microorganisms on surfaces and interfaces as a biofilm is very common and plays important role in various areas such as material science, biomedicine, or waste treatment among others. Due to their inhomogeneous structure and the variance in the microorganism consortium, the analysis of biofilms represents a significant challenge. An online fluorescence sensor was developed that is able to measure the most important biological fluorophores (proteins, nicotinamide adenine dinucleotide, and flavin) in a noninvasive manner in biofilms, e.g. in bioelectrochemical applications. The sensor gives the opportunity to continuously draw conclusions on the metabolic state of the biofilm. The developed sensor has a diameter of 1 mm at the sensor tip and can be moved on and into the biofilm surface. In the first experiment, the measuring range of the sensor and the long‐term stability could be determined and the system applicability was confirmed. In addition, measurements in biofilm‐like structures could be performed. The formation of a wastewater‐based biofilm was monitored using the developed sensor, demonstrating the functionality of the sensor in a proof‐of‐principle experiment.

中文翻译:

用于生物膜及其微环境在线监测的光纤荧光传感器的开发和表征

微生物在表面和界面上作为生物膜的生长非常普遍,在材料科学、生物医学或废物处理等各个领域发挥着重要作用。由于它们的不均匀结构和微生物群落的差异,生物膜的分析是一项重大挑战。开发了一种在线荧光传感器,它能够以非侵入性方式测量生物膜中最重要的生物荧光团(蛋白质、烟酰胺腺嘌呤二核苷酸和黄素),例如在生物电化学应用中。传感器提供了对生物膜的代谢状态不断得出结论的机会。开发的传感器在传感器尖端的直径为 1 毫米,可以在生物膜表面上移动。在第一个实验中,确定了传感器的测量范围和长期稳定性,确认了系统的适用性。此外,还可以对生物膜样结构进行测量。使用开发的传感器监测基于废水的生物膜的形成,在原理验证实验中证明了传感器的功能。
更新日期:2020-03-02
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