This study assessed the efficiency of cervical relaxation protocol using none, half or full dose (1.0 mg) of oestradiol benzoate in Dorper ewes subjected to non‐surgical embryo recovery (NSER). Thirty‐six pluriparous ewes received progestogen sponge (60 mg) for 9 days plus eCG administration (300 IU i.m.) 24 hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16 hr before NSER 37.5 µg d‐cloprostenol i.m. and different doses of oestradiol benzoate: 0.0 mg (0EB group; n  = 12); 0.5 mg (0.5EB group; n  = 12) or 1.0 mg of oestradiol (1.0EB group, n  = 12). All ewes received oxytocin (50 IU) i.v. 20 min before NSER, which was performed 8 days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24 hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post‐NSER fertility. NSER was performed in 91.7% (33/36) of the animals with overall fluid recovery efficiency over 97% (p  > .05). The cervical transposing with Hegar dilator was longer (p  < .05) in 0EB (4.2 ± 0.3 min) compared to 0.5EB (1.7 ± 0.3 min) and 1.0EB group (1.5 ± 0.3 min). The cervical transposing with mandrel/catheter was longer (p  < .05) in 0EB (2.4 ± 0.5 min) than 1.0EB group (1.3 ± 0.5 min). Overall duration of uterine flushing was 25.4 min with structure recovery rate of 43.5%, with no difference among groups (p  > .05). The post‐NSER fertility was higher (p  < .05) in 0.0EB (90%) than 0.5EB group (36.4%). In conclusion, NSER can be successfully performed in Dorper ewes by using a cervical relaxation protocol without oestradiol benzoate.

中文翻译：

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在Dorper母羊的宫颈松弛方案中，可以在不使用或降低剂量的雌二醇苯甲酸酯的情况下成功进行宫颈经子宫冲洗。

这项研究评估了在接受非手术胚胎恢复（NSER）的多珀母羊中，不使用，半剂量或全剂量（1.0 mg）的雌二醇苯甲酸酯对颈椎放松方案的有效性。三十六只母羊母羊在摘除海绵前24小时接受孕激素海绵（60 mg）治疗9天，并给予eCG（300 IU im）。母羊不交配，随机分配在NSER 37.5 µg d-氯前列醇im和不同剂量的雌二醇苯甲酸酯：0.0 mg（0EB组；n  = 12）之前的16小时接受。0.5 mg（0.5EB组; n  = 12）或1.0 mg雌二醇（1.0EB组，n = 12）。NSER之前20分钟，所有母羊均接受催产素（50 IU），这是在去除海绵后8天进行的。在NSER前24小时，通过直肠超声对黄体进行计数。程序完成后，母羊处于自然繁殖期，以检查其NSER后的繁殖力。在91.7％（33/36）的动物中进行了NSER，总体液回收率超过97％（p  > .05）。 在0EB（4.2±0.3分钟）中，用Hegar扩张器进行宫颈移位的时间更长（p <.05），而在0.5EB（1.7±0.3分钟）和1.0EB组（1.5±0.3分钟）中则更长。带芯轴/导管的宫颈移位更长（p 0EB（2.4±0.5分钟）比1.0EB组（1.3±0.5分钟）<0.05。子宫冲洗的总时间为25.4分钟，结构恢复率为43.5％，各组之间无差异（p  > .05）。 0.0EB（90％）的NSER后生育率高于0.5EB组（36.4％）（p <.05）。综上所述，通过使用宫颈舒张方案而不使用雌二醇苯甲酸酯，NSER可以在Dorper母羊中成功进行。