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Stability of the resistance to lambda‐cyhalothrin in the ladybird beetle Eriopis connexa
Entomologia Experimentalis et Applicata ( IF 1.9 ) Pub Date : 2020-05-11 , DOI: 10.1111/eea.12911
Alice Sutana Rodrigues 1 , Raul Narciso Carvalho Guedes 2 , Herbert Álvaro Abreu de Siqueira 1 , Jorge Braz Torres 1
Affiliation  

Natural enemies resistant to insecticides are expected to help control the remaining arthropod pests after insecticide application and, hence, prevent crop damage, pest resurgence, and resistance selection. Field‐evolved resistance to lambda‐cyhalothrin and to other pyrethroids exhibited by the neotropical ladybird beetle Eriopis connexa (Germar) (Coleoptera: Coccinellidae) has been characterized and enhanced under laboratory selection. In this study, we investigated the stability of the resistance and its relationship with detoxification enzyme activity and biological performance, which are important for a biocontrol agent. One subgroup of the resistant population of E. connexa was established without selection pressure (R‐UNSEL) during eight generations, and compared to either a susceptible (SUS) or its parental resistant (R‐SEL) population. The resistance ratio in R‐UNSEL was reduced by 50% compared to R‐SEL between the first and fourth generation without selection pressure, but stayed stable afterwards, from the fifth to the eighth generation. Despite eight generations without selection pressure and reduction in the resistance level, the resistance ratio in R‐UNSEL was still 39× greater than in the SUS population. The reduced resistance in R‐UNSEL correlated to reduction in esterase activity, but the R‐UNSEL maintained greater activity than the SUS group. The absence of selection pressure and reduction in enzyme activity in R‐UNSEL did not mitigate the adaptive costs, with 2.7× lower egg production compared to SUS females. These findings indicate that resistance to lambda‐cyhalothrin in R‐UNSEL has already stabilized in the population. Although the resistance ratio and detoxifying enzymes were reduced in R‐UNSEL, the impact on fecundity was maintained. Furthermore, the absence of crossing with wild SUS individuals will allow the R‐UNSEL offspring to retain the resistance allowing survival to lambda‐cyhalothrin even when used at the highest recommended field rate.

中文翻译:

瓢虫甲虫Eriopis connexa对λ-三氯氟氰菊酯抗性的稳定性

对杀虫剂具有抗性的天敌有望在杀虫剂施用后帮助控制剩余的节肢动物害虫,从而防止作物损害、害虫死灰复燃和抗性选择。新热带瓢虫甲虫 Eriopis connexa (Germar)(鞘翅目:瓢虫科)的田间进化对 λ-三氯氟氰菊酯和其他拟除虫菊酯的抗性已在实验室选择下得到表征和增强。在这项研究中,我们研究了抗性的稳定性及其与解毒酶活性和生物学性能的关系,这对生物防治剂很重要。E. connexa 抗性种群的一个亚组是在八代没有选择压力 (R-UNSEL) 的情况下建立的,并与易感 (SUS) 或其亲本抗性 (R-SEL) 种群进行比较。在没有选择压力的第一代和第四代之间,R-UNSEL 中的电阻率比 R-SEL 降低了 50%,但之后从第五代到第八代保持稳定。尽管 8 代没有选择压力且抗性水平降低,但 R-UNSEL 中的抗性比仍然比 SUS 群体高 39 倍。R-UNSEL 抗性降低与酯酶活性降低相关,但 R-UNSEL 保持比 SUS 组更高的活性。R-UNSEL 中没有选择压力和酶活性降低并没有降低适应性成本,与 SUS 雌性相比产蛋量低 2.7 倍。这些发现表明 R-UNSEL 中对 λ-三氯氟氰菊酯的耐药性已经在人群中稳定下来。虽然R-UNSEL中抗性比和解毒酶降低,但对繁殖力的影响保持不变。此外,不与野生 SUS 个体杂交将允许 R-UNSEL 后代保留抵抗力,即使在推荐的最高田间使用率下也能存活对 λ-三氯氟氰菊酯。
更新日期:2020-05-11
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