This study was aimed to evaluate the efficiency of the organic chlorine and acetic acid solutions on the inactivation of adhered cells of Escherichia coli, Cronobacter sakazakii and Klebsiella pneumoniae to lettuce. Besides, the growth and inactivation of K. pneumoniae adhered to lettuce was modeled. According to the findings, the use of chlorine solution (170 mg/ml of total residual chlorine) caused reductions of 1.8, 1.9, and 1.9 log for E. coli, C. sakazakii, and K pneumoniae, respectively, were recorded. In this regard, the organic chloramine was more effective in controlling the adhered microorganisms while compared with 1.5% acetic acid solution, while the addition of 0.5% sodium chloride to 1.5% acetic acid solution increased microbial inactivation. K. pneumoniae RC‐34 inactivation was characterized by the presence of two sub‐populations with different resistances against the proposed sanitizers. Moreover, the growth kinetic parameters of K. pneumoniae RC‐34 adhered to lettuce leaves were very similar to that reported in the literature for nonadhered microorganisms. The predictive data generated can be valuable to assess the growth and inactivation of produce adhered microorganisms in leafy produce.

中文翻译：

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使用有机氯和乙酸溶液对微生物进行灭活建模并估计肠杆菌科细菌对生菜的生长动力学（Lactuca sativa L.）

这项研究旨在评估有机氯和乙酸溶液对大肠杆菌，阪崎肠杆菌和肺炎克雷伯菌的粘附细胞失活的抑制作用。此外，还模拟了粘附在生菜上的肺炎克雷伯菌的生长和失活。根据调查结果，使用氯溶液（总残留氯170 mg / ml）可使大肠杆菌，阪崎肠杆菌和肺炎克雷伯菌减少1.8、1.9和1.9 log 。分别进行记录。在这方面，与1.5％的乙酸溶液相比，有机氯胺在控制粘附的微生物上更有效，而在1.5％的乙酸溶液中添加0.5％的氯化钠则增加了微生物的灭活作用。肺炎克雷伯菌RC-34失活的特征是存在两个亚群，对拟议的消毒剂具有不同的抗药性。此外，粘附在生菜叶片上的肺炎克雷伯菌RC-34的生长动力学参数与非粘附微生物文献中报道的非常相似。产生的预测数据对于评估多叶产品中附着的微生物的生长和灭活可能是有价值的。