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Fate of Listeria on various food contact and noncontact surfaces when treated with bacteriophage
Journal of Food Safety ( IF 2.4 ) Pub Date : 2020-02-11 , DOI: 10.1111/jfs.12775
Robert G. Reinhard 1, 2 , Robin M. Kalinowski 2 , Peter W. Bodnaruk 2 , Joseph D. Eifert 1 , Renee R. Boyer 1 , Susan E. Duncan 1 , R. Hartford Bailey 3
Affiliation  

Study objective was to determine efficacy of a bacteriophage suspension against Listeria spp. when applied to three common types of materials used in food manufacturing facilities. Materials included two food contact materials (stainless steel and polyurethane thermoplastic belting) and one noncontact material (epoxy flooring). Coupons of each material were inoculated with a cocktail containing L. monocytogenes and L. innocua (4 to 5‐log₁₀ CFU/cm²). Two phage concentrations and a control, 0, 2 × 10⁷ and 1 × 10⁸ PFU/cm² were evaluated. Treated samples were held at 4 or 20°C for 1 and 3 hr to determine the effect of temperature and treatment time. Reductions in Listeria populations ranged from 1.27 to 3.33 log₁₀ CFU/cm² on stainless steel, from 1.17 to 2.76 log₁₀ CFU/cm² on polyurethane thermoplastic belting, and from 1.19 to 1.76 log₁₀ CFU/cm² on epoxy resin flooring. Higher phage concentration (1 × 10⁸ PFU/cm²), longer treatment time (3 hr), and processing area temperature of 20°C showed a greater (p ≤ .05) reduction of Listeria on the stainless‐steel and polyurethane thermoplastic belting coupons. Overall, Listeria reduction by phage treatment occurred on all three materials tested, under all conditions.

中文翻译:

用噬菌体处理后,李斯特菌在各种食品接触和非接触表面上的命运

研究目的是确定噬菌体悬浮液对李斯特菌的功效。当应用于食品制造设施中使用的三种常见材料时。材料包括两种食品接触材料(不锈钢和聚氨酯热塑性带)和一种非接触材料(环氧树脂地板)。每种材料的样品都接种了含有单核细胞增生李斯特菌和无害李斯特菌(4 到 5-log₁₀ CFU/cm²)的混合物。评估了两个噬菌体浓度和一个对照,0、2 × 10⁷ 和 1 × 10⁸ PFU/cm²。处理过的样品在 4 或 20°C 下保持 1 小时和 3 小时,以确定温度和处理时间的影响。李斯特菌种群减少范围为不锈钢上的 1.27 至 3.33 log₁₀ CFU/cm²,聚氨酯热塑性传送带上的 1.17 至 2.76 log₁₀ CFU/cm²,以及 1.19 至 1。环氧树脂地板上 76 log₁₀ CFU/cm²。更高的噬菌体浓度 (1 × 10⁸ PFU/cm²)、更长的处理时间 (3 小时) 和 20°C 的加工区温度表明不锈钢和聚氨酯热塑性带状试样上李斯特菌的减少幅度更大 (p ≤ .05) . 总体而言,在所有条件下,所有三种测试材料均通过噬菌体处理减少了李斯特菌。
更新日期:2020-02-11
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