This study was conducted to investigate the effect of grape seed procyanidins (GSP) on growth performance, digestive enzyme activity, antioxidant enzyme activity and mRNA expression in weanling piglets. A total of 96 piglets (Pietrain × Large White) with an average initial body weight (BW) of 8.4 ± 1.7 kg were weaned at 28 days, and randomly divided into 4 groups. Four groups of animals were fed with a basic diet supplemented with various doses of GSP (0, 40, 70 and 100 mg/kg respectively) during the 28‐day treatment period. The results showed that the group receiving 40 mg/kg GSP significantly increased the average daily gain (ADG, p  < .05) and decrease the feed/gain ratio (F/G, p  < .05). Interestingly, the incidence of diarrhoea was significantly reduced in the groups of 40 and 70 mg/kg GSP, but it was increased in the group of 100 mg/kg GSP. Subsequent biochemical studies indicated that dietary GSP significantly increased the activities of digestive enzymes and antioxidant enzymes, including amylase (Amy), lipase(LPS, p  < .05), glutathione peroxidase activity (GSH‐Px, p  < .05), superoxide dismutase activity (SOD, p  < .05) and total antioxidant capacity (T‐AOC, p  < .05) in serum, liver and muscle, increased the expression of GSH‐Px, SOD and CAT genes (p  < .05) in the liver, and decreased the level of malondialdehyde (MDA, p  < .05) in serum, liver and muscle. Taken together, these studies revealed that low GSP supplement in diets can improve growth performance of weaned piglets, which is associated with increased digestive and antioxidant enzyme activities and enhanced resistance to weanling stress.

中文翻译：

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饮食中的葡萄籽原花青素通过改善断奶仔猪的抗氧化酶活性和mRNA表达来抑制断奶应激。

这项研究旨在研究葡萄籽原花青素（GSP）对断奶仔猪生长性能，消化酶活性，抗氧化酶活性和mRNA表达的影响。在第28天，对平均初始体重（BW）为8.4±1.7千克的96头仔猪（Pietrain×大白猪）断奶，并随机分为4组。在28天的治疗期内，四组动物接受了基本饮食，并补充了各种剂量的GSP（分别为0、40、70和100 mg / kg）。结果表明，接受40 mg / kg GSP的组显着提高了平均日增重（ADG，p  <.05），并降低了饲料/增重比（F / G，p <.05）。有趣的是，在40和70 mg / kg GSP的组中，腹泻的发生率显着降低，但是在100 mg / kg GSP的组中，腹泻的发生率显着增加。随后的生化研究表明，饮食中的GSP可显着提高消化酶和抗氧化酶的活性，包括淀粉酶（Amy），脂肪酶（LPS，p  <.05），谷胱甘肽过氧化物酶活性（GSH-Px，p  <.05），超氧化物歧化酶 血清，肝和肌肉中的活性（SOD，p  <.05）和总抗氧化能力（T-AOC，p <.05），增加了谷胱甘肽过氧化物酶，谷胱甘肽过氧化物酶，过氧化物酶和CAT基因的表达（p  <0.05）。肝脏，并降低了丙二醛（MDA，p <.05）在血清，肝脏和肌肉中。总之，这些研究表明，日粮中添加低GSP可以改善断奶仔猪的生长性能，这与增加的消化和抗氧化酶活性以及增强的对断奶压力的抵抗力有关。