Distinguishing hatchery‐reared fishes from wild conspecifics can be required to quantify the success of augmentation programmes. This study estimated the probability of identifying calcein‐marked, hatchery‐reared Colorado pikeminnow Ptychocheilus lucius Girard from external and internal structures. Both control and marked fish held in the laboratory were correctly identified 80% of the time after 300 days. A fluorometer was more accurate 180 days post‐marking, whereas visual observation was more discriminating at 300 days. There were no differences in detection rates among structures in laboratory fish, and for most structures of fish held outdoors, detection rates were <20%. Overall, a strong negative effect of light on mark persistence was observed. Also, an initial positive effect of fish size on mark fluorescence and a negative effect of fish growth were detected. These results suggest the potential use of calcein as a batch‐marking tool would be aided by future studies that better quantify light exposure and calcein mark persistence in both field and experimental settings.

中文翻译：

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濒危Colorado pikeminnow Ptychocheilus lucius荧光钙黄绿素标记保留和鉴定的功效

可能需要将孵化场养殖的鱼类与野生同种鱼类区分开来，以量化扩增计划的成功。本研究估计了从外部和内部结构识别出标记有钙黄绿素、孵化场饲养的科罗拉多梭鱼 Ptychocheilus lucius Girard 的可能性。300 天后，实验室中的对照鱼和标记鱼的正确识别率为 80%。荧光计在标记后 180 天更准确，而目视观察在 300 天时更具辨别力。实验室鱼的结构之间的检出率没有差异，对于大多数在户外饲养的鱼的结构，检出率<20%。总体而言，观察到光对标记持久性的强烈负面影响。还，检测到鱼大小对标记荧光的初步积极影响和鱼生长的负面影响。这些结果表明，钙黄绿素作为批量标记工具的潜在用途将得到未来研究的帮助，这些研究可以更好地量化野外和实验环境中的光照和钙黄绿素标记持久性。