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Sex identification PCR–RFLP assay tested in eight species of Sebastes rockfish
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2020-04-29 , DOI: 10.1007/s12686-020-01150-y
Felix Vaux , Hannah M. Aycock , Sandra Bohn , Leif K. Rasmuson , Kathleen G. O’Malley

The phenotypic identification of sex in Sebastes rockfish is difficult and often impractical from a management perspective, and the genetic basis of sex determination in the genus is currently uncertain. We tested a previously developed sex identification polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) assay in eight species of Sebastes rockfish. Results indicated that the association of this restriction site with sex is species-dependent.



中文翻译:

在八种塞巴斯蒂斯石鱼中进行了性别鉴定PCR-RFLP分析

从管理的角度来看,塞巴斯蒂斯石斑鱼中性别的表型鉴定是困难的,并且通常是不切实际的,并且目前尚不确定该属中性别确定的遗传基础。我们在八种塞巴斯蒂斯石鱼中测试了先前开发的性别鉴定聚合酶链反应限制片段长度多态性(PCR-RFLP)分析。结果表明该限制性位点与性别的关联是物种依赖性的。

更新日期:2020-04-29
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