Abstract—rDNA genes play an important role in epigenetic regulation and in differentiation of eukaryotic cells. Using the 4C (circular chromosome conformation capture) approach and model HEK293T cells, we analyzed the rDNA-contacting gene FANK1, using anchor located inside rDNA genes. At the 5' end of the FANK1 gene we detected frequent contacts with rDNA clusters. The contact sites coincide with the border where chromatin state changes and nucleosome positioning. The adjacent genes DHX32, BCCIP and UROS are located in the active chromatin and are transcribed, but do not contact with rDNA genes, while FANK1 gene is silenced, and is located in repressed chromatin. Heat shock treatment dramatically changes the pattern of rDNA contacts in the region and induces about 4-fold increase in activation of the FANK1 gene. We conclude that rDNA contacts may be involved in repression of the FANK1 gene.

中文翻译：

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具有FANK1基因的rDNA簇的接触位点对应于抑制的染色质

摘要—rDNA基因在表观遗传调控和真核细胞分化中起着重要作用。使用4C（圆形染色体构象捕获）方法和HEK293T细胞模型，我们使用位于rDNA基因内部的锚点分析了rDNA接触基因FANK1。在FANK1基因的5'末端，我们检测到与rDNA簇的频繁接触。接触位点与染色质状态改变和核小体定位的边界重合。相邻基因DHX32，BCCIP和UROS位于活性染色质中并被转录，但不与rDNA基因接触，而FANK1该基因被沉默，位于被抑制的染色质中。热激处理极大地改变了该区域中rDNA的接触方式，并诱导了FANK1基因的激活增加了约4倍。我们得出的结论是，rDNA接触可能与FANK1基因的抑制有关。