Abstract

A synthetic genetic chain was created—a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.

中文翻译：

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基于TET-ON 3G反式激活剂的强力霉素敏感双促进剂积分器

摘要

创建了一条合成的遗传链-一个两个启动子的整合子，其输出信号的活性仅在两个启动子在该链的输入端具有高活性的情况下才具有很高的活性。所述整合子通过人类存活蛋白和端粒酶基因的癌症特异性启动子的实例显示可工作，所述癌症特异性启动子可用于肿瘤细胞中靶基因的特异性转录。为了创建积分器，我们使用了由强力霉素调节的著名的TET-ON 3G反式激活剂。