The objective of this study was to evaluate the synthesis, structural analysis, and biological effects of novel ((2, 4-dioxothiazolidin-5-ylidene)methyl)phenyl derivatives. The efficacy of 15-PGDH inhibition increased for the substituents in the derivatives in the order: cyclohexylpropyl > cyclohexylethyl > cyclohexylmethyl > cyclohexyl. Compound 12 inhibited 15-PGDH activity by binding to the amino acids Ile 214, Ile 210, Ile 194, Gln 148, and Leu 191 of 15-PGDH. Compounds 4, 22, and 23 produced the highest increment in PGE₂ concentration, which was 122.19, 100.14, and 206.80%, respectively, compared to that of the control. Also, compounds 38 and 39, in which the central phenyl ring and the 2, 4-thiazolidinedione moiety were linked by a single bond, produced a relatively high increment in PGE₂ concentration, which was 106.81 and 118.66%, respectively, compared to that of the control. The wound closure rates of compounds 22 and 39 were the highest, being 247.56 and 202.42%, respectively, compared to that of the positive control. Therefore, compounds 22 and 39 could not only efficiently regulate PGE₂ concentration, but also induce cellular regeneration, and are expected to effectively treat a variety of diseases resulting from PGE₂ deficiency.

这项研究的目的是评估新型（（2，4-dioxothiazolidin-5-ylidene）methyl）phenyl衍生物的合成，结构分析和生物学效应。对于衍生物中的取代基，抑制15-PGDH的功效按以下顺序增加：环己基丙基>环己基乙基>环己基甲基>环己基。化合物12通过与15-PGDH的氨基酸Ile 214，Ile 210，Ile 194，Gln 148和Leu 191结合而抑制15-PGDH活性。化合物4,22和23所产生的PGE最高增量₂浓度，其分别为122.19，100.14，206.80和％，相对于对照的。另外，化合物38和39，其中中央苯环和2,4-噻唑烷二酮部分通过单键连接，与对照相比，PGE _2的浓度相对较高，分别为106.81和118.66％。与阳性对照相比，化合物22和39的伤口闭合率最高，分别为247.56和202.42％。因此，化合物22和39不仅可以有效地调节PGE _2的浓度，而且还可以诱导细胞再生，并有望有效治疗由于PGE ₂缺乏引起的多种疾病。