Streptomyces avermitilis MA4680 was used for the biotransformation of phloretin, one of the dihydrochalcone found in apple bark. After LC analysis, two main biotransformed products were identified and they were further analyzed using GC/MS. After BSTFA derivatization of biotransformed product, they were interpreted as regioselectively hydroxylated products of phloretin in Bring. Maximum conversion of phloretin was 6.7% with 1 h of reaction, and the phloretin and reaction products were completely metabolized after 3 h of reaction due to cellular metabolism. Addition of 0.5 mM quinidine completely blocked hydroxylation of phloretin, which means the hydroxylation proceed by P450 monoxygenase dependent metabolism. Addition of Brij 35 detergent resulted in 150% increase of hydroxylated product due to facilitating transport of phloretin and its reaction product across cellular membrane.

阿维链霉菌MA4680用于促性腺激素的生物转化，这是苹果树皮中发现的二氢查耳酮之一。LC分析后，鉴定了两种主要的生物转化产物，并使用GC / MS进行了进一步分析。BSTFA对生物转化产物进行衍生化后，在Bring中将其解释为视黄素的区域选择性羟基化产物。在反应1小时后，伞菌素的最大转化率为6.7％，反应3小时后，由于细胞的新陈代谢，伞菌素和反应产物被完全代谢。添加0.5 mM奎尼丁可完全阻断phloretin的羟基化，这意味着羟基化是通过P450单加氧酶依赖性代谢进行的。