This study examined the effect of chitosan elicitor with four different concentrations (0, 0.2, 0.4 and 0.6 g/l) on physiological and biochemical properties of stevia under four levels of salinity stress (0, 50, 100, 150 mM level of NaCl). Salt stress caused reduction of chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll, carotenoid and total protein content. The increment of malondialdehyde (MDA) content was not significant in all NaCl levels, while the CAT and POX activities were increased as well as stevioside and rebaudioside A under salinity stress. On one side, chitosan treatments could compensate the reduction of physiological traits such as photosynthetic pigments and protein content. On the other side, chitosan caused multiple increases in malondialdehyde content, antioxidant enzymes activity (catalase and peroxidase), steviol glycosides (stevioside and rebaudioside A) under salt stress. We report for the first time, the potential of chitosan to enhance salinity-tolerant abilities in stevia through increment of the salt-adaptive factors and to diminish harmful damages caused by NaCl stress.

中文翻译：

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甜叶菊 Bertoni 对盐胁迫和壳聚糖诱导子的反应。

本研究考察了四种不同浓度（0、0.2、0.4和0.6 g/l）的壳聚糖诱导剂对四种盐度胁迫水平（0、50、100、150 mM NaCl水平）下甜叶菊生理生化特性的影响。 。盐胁迫导致叶绿素a（Chl a）、叶绿素b（Chl b）、总叶绿素、类胡萝卜素和总蛋白质含量减少。盐度胁迫下，丙二醛（MDA）含量增加并不显着，而CAT和POX活性以及甜菊苷和莱鲍迪甙A活性均增加。一方面，壳聚糖处理可以补偿光合色素和蛋白质含量等生理特性的降低。另一方面，壳聚糖在盐胁迫下导致丙二醛含量、抗氧化酶活性（过氧化氢酶和过氧化物酶）、甜菊糖苷（甜菊苷和莱鲍迪甙A）的倍数增加。我们首次报道了壳聚糖通过增加盐适应性因子来增强甜叶菊耐盐能力并减少 NaCl 胁迫造成的有害损害的潜力。