Market is increasingly demanding vegetables with high quality and nutraceutical characteristics. It was demonstrated that leafy vegetables can get benefit from biostimulants, for the reduction of nitrate concentration and the increment of antioxidants, with potential benefit for human health. The research purpose was to investigate on the role of a novel plant-based biostimulant in affecting nitrogen and carbon metabolism in wild rocket (Diplotaxis tenuifolia L.). Foliar spray treatments were performed with extracts obtained from borage (Borago officinalis L.) leaves and flowers. To evaluate the treatments effect, in vivo determinations (chlorophyll a fluorescence and chlorophyll content) were performed. At harvest, nitrate concentration, sucrose, total sugars, chlorophyll, and carotenoids levels were measured in leaves. In order to characterize the mechanism of action also at molecular level, a set of genes encoding for some of the key enzymes implicated in nitrate and carbon metabolism was selected and their expression was measured by qRT-PCR. Interesting results concerned the increment of sucrose, coherent with a high value of Fv/Fm, in addition to a significant reduction of nitrate and ABA than control, and an enhanced NR in vivo activity. Also, genes expression was influenced by extracts, with a more pronounced effect on N related genes.

中文翻译：

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琉璃苣提取物会影响野外火箭的质量并影响硝酸盐和碳的代谢

市场对具有高质量和营养特性的蔬菜的要求越来越高。事实证明，多叶蔬菜可从生物刺激剂中受益，减少硝酸盐浓度和增加抗氧化剂，对人体健康具有潜在益处。研究目的是研究一种新型的植物性生物兴奋剂在影响野生火箭（Diplotaxis tenuifolia L.）中氮和碳代谢中的作用。用从琉璃苣（Borago officinalis L.）叶和花中提取的提取物进行叶面喷雾处理。为了评估治疗效果，在体内进行了测定（叶绿素a荧光和叶绿素含量）。在收获时，测量叶片中的硝酸盐浓度，蔗糖，总糖，叶绿素和类胡萝卜素水平。为了在分子水平上也表征作用机理，选择了一组编码一些涉及硝酸盐和碳代谢的关键酶的基因，并通过qRT-PCR测量了它们的表达。有趣的结果涉及蔗糖的增加，与Fv / Fm的高值相符，此外硝酸盐和ABA的含量比对照显着降低，以及NR体内活性增强。同样，基因表达受到提取物的影响，对N相关基因的影响更为明显。