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Effects of combining Renlon with Renbarb1 and Renbarb2 on resistance of cotton (Gossypium hirsutum L.) to reniform nematode (Rotylenchulus reniformis Linford and Oliveria)
Euphytica ( IF 1.9 ) Pub Date : 2020-04-01 , DOI: 10.1007/s10681-020-02580-3
Amanda G. Gaudin , Ted P. Wallace , Jodi A. Scheffler , Salliana R. Stetina , Martin J. Wubben

The upland cotton (Gossypium hirsutum L.) germplasm lines BARBREN-713, having the Renbarb1 and Renbarb2 quantitative trait loci (QTL), and LONREN, carrying the Renlon QTL, have introgressed resistance to reniform nematode (Rotylenchulus reniformis Linford and Oliveria). Stunting has been observed in the LONREN source of resistance when subjected to heavy nematode pressure but not for BARBREN-713. To investigate the potential to reduce stunting in the LONREN source of resistance, cotton lines were developed with different combinations of reniform nematode resistance QTL from LONREN and BARBREN-713. Marker assisted selection was conducted to identify the following QTL combinations: (1) Renbarb1 + Renbarb2, (2) Renbarb1 + Renbarb2 + Renlon, (3) Renbarb2 + Renlon, (4) Renbarb1 + Renlon, (5) Renbarb1 alone, (6) Renbarb2 alone, (7) Renlon alone, and (8) a Null susceptible genotype. QTL combinations were inoculated with 0, 2300, or 5000 reniform nematodes per 120 cm3 soil in growth chamber tests. Plants were harvested at 60 days after inoculation, plant growth measurements made, and the nematode reproduction factor calculated. In this study, the Renbarb1 genotype was scored as susceptible to reniform nematode. Combining Renlon with Renbarb2 did not improve resistance to reniform nematode; however, the Renbarb2/Renlon combination showed improved growth compared to LONREN-1, BARBREN-713, and the susceptible null check.

中文翻译:

Renlon与Renbarb1和Renbarb2组合对棉花(Gossypium hirsutum L.)对肾形线虫(Rotylenchulus reniformis Linford and Oliveria)抗性的影响

陆地棉 (Gossypium hirsutum L.) 种质系 BARBREN-713 具有 Renbarb1 和 Renbarb2 数量性状基因座 (QTL),而 LONREN 携带 Renlon QTL,对肾形线虫 (Rotylenchulus reniformis Linford 和 Oliveria) 具有渐渗抗性。当受到重线虫压力时,在 LONREN 抗性源中观察到发育迟缓,但在 BARBREN-713 中未观察到。为了研究减少 LONREN 抗性来源发育迟缓的潜力,开发了具有来自 LONREN 和 BARBREN-713 的肾状线虫抗性 QTL 的不同组合的棉花品系。进行标记辅助选择以鉴定以下 QTL 组合:(1) Renbarb1 + Renbarb2,(2) Renbarb1 + Renbarb2 + Renlon,(3) Renbarb2 + Renlon,(4) Renbarb1 + Renlon,(5) Renbarb1 单独,(6) ) Renbarb2 单独,(7) Renlon 单独,(8) Null 易感基因型。在生长室测试中,QTL 组合在每 120 cm3 土壤中接种 0、2300 或 5000 条肾形线虫。在接种后 60 天收获植物,进行植物生长测量,并计算线虫繁殖因子。在这项研究中,Renbarb1 基因型被评为对肾状线虫敏感。Renlon 与 Renbarb2 结合使用并没有提高对肾状线虫的抗性;然而,与 LONREN-1、BARBREN-713 和易感无效检查相比,Renbarb2/Renlon 组合显示出更好的生长。在这项研究中,Renbarb1 基因型被评为对肾状线虫敏感。Renlon 与 Renbarb2 结合使用并没有提高对肾状线虫的抗性;然而,与 LONREN-1、BARBREN-713 和易感无效检查相比,Renbarb2/Renlon 组合显示出更好的生长。在这项研究中,Renbarb1 基因型被评为对肾状线虫敏感。Renlon 与 Renbarb2 结合使用并没有提高对肾状线虫的抗性;然而,与 LONREN-1、BARBREN-713 和易感无效检查相比,Renbarb2/Renlon 组合显示出更好的生长。
更新日期:2020-04-01
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