The use of iron-efficient rootstocks can be a sustainable way to enhance iron (Fe) fruit content in tomato. A hybrid tomato variety was grafted on a genotyped population of recombinant inbred lines derived from Solanum pimpinellifolium, and compared with self- and non-grafted controls under low iron (5.2 µM) growing conditions. Rootstock effects on total fruit yield, fruit [Fe] and yield Fe content (FeUEc) were the target traits; other minerals were also investigated by quantitative trait loci (QTL) and candidate gene analyses. The rootstock genotype affected fruit concentrations of Fe, Ca, Mg, Mn, Na, P, S, Si and Al. Most rootstocks increased FeUEc. Fruit and leaf [Fe] and FeUEc were genetically complex, involving epistatic interactions. Six and eight QTLs were detected for these traits, respectively, by multiple QTL mapping. Two kinds of relevant genes were found among candidates within QTLs for iron related traits: those coding for secretory proteins specific of the tomato xylem sap under iron deficiency, and others having a role in iron uptake such as TOMLHA1, FRO1, NRAMP2, FER and MYB72. Detected QTLs might reflect differences in the regulatory regions of those relevant genes, more than in their coding sequences.

中文翻译：

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低铁胁迫下砧木介导的番茄果实产量和品质性状的QTL和候选基因分析

使用铁效率高的砧木可以是提高番茄中铁 (Fe) 含量的可持续方法。将杂交番茄品种嫁接在源自 Solanum pimpinellifolium 的重组自交系的基因分型群体上，并在低铁 (5.2 µM) 生长条件下与自嫁和非嫁接对照进行比较。砧木对果实总产量、果实[Fe]和产量Fe含量（FeUEc）的影响是目标性状；还通过数量性状位点 (QTL) 和候选基因分析研究了其他矿物质。砧木基因型影响果实中铁、钙、镁、锰、钠、磷、硫、硅和铝的浓度。大多数砧木增加了 FeUEc。果实和叶子 [Fe] 和 FeUEc 是遗传复杂的，涉及上位相互作用。通过多重 QTL 定位，分别检测到这些性状的 6 个和 8 个 QTL。在与铁相关性状的 QTL 候选中发现了两种相关基因：编码缺铁条件下番茄木质部汁液特异性分泌蛋白的基因，以及其他在铁吸收中起作用的基因，如 TOMLHA1、FRO1、NRAMP2、FER 和 MYB72 . 检测到的 QTL 可能反映了这些相关基因调控区域的差异，而不是它们的编码序列。