当前位置: X-MOL 学术J. Environ. Qual. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Fluorine and white clover: Assessing F's impact on Rhizobium leguminosarum
Journal of Environmental Quality ( IF 2.4 ) Pub Date : 2020-06-11 , DOI: 10.1002/jeq2.20089
Thangavelautham Geretharan 1, 2 , Paramsothy Jeyakumar 1 , Michael Bretherton 1 , Christopher W. N. Anderson 1
Affiliation  

The soil fluorine (F) concentration in New Zealand agricultural soils has increased with time as a direct result of the widespread application of phosphate fertilizer to land. Elevated soil F concentrations may potentially harm soil microorganisms, which are important for nutrient cycling and soil formation. Rhizobium leguminosarum is a N2 -fixing soil bacterium that is a fundamental component in New Zealand legume-based pastoral farming. Any impact of F on Rhizobium leguminosarum would have an adverse effect on New Zealand pasture production. In this study, F toxicity to Rhizobium leguminosarum was examined as a first step to develop F guideline values for New Zealand agricultural soils. Bottle-based experiments were conducted to examine the effect of the F- ion on Rhizobium-white clover (Trifolium repens L.) symbiosis by observing nodule morphology and growth. Results indicate that the F- concentration that causes 10% inhibition of Rhizobium respiration (IC10 ) for F- toxicity to Rhizobium leguminosarum was >100 mg F- L-1 . Significant morphological changes occurred when Rhizobium was exposed to F concentrations of 500 and 1000 mg L-1 . Both light and transmission electron micrographs confirmed that the Rhizobium leguminosarum-white clover interaction was not influenced by F- concentrations >100 mg L-1 . The toxic F- concentration for Rhizobium leguminosarum determined in this study is orders of magnitude higher than the F- concentration in New Zealand agriculture soils under "normal conditions." There appears to be no indication of imminent risk of soil F to Rhizobium leguminosarum.

中文翻译:

氟和白三叶草:评估 F 对豆科根瘤菌的影响

作为磷肥广泛应用于土地的直接结果,新西兰农业土壤中的土壤氟 (F) 浓度随着时间的推移而增加。土壤 F 浓度升高可能会危害土壤微生物,这对养分循环和土壤形成很重要。豆类根瘤菌是一种固氮土壤细菌,是新西兰豆类牧区的基本组成部分。F 对豆根瘤菌的任何影响都会对新西兰牧草生产产生不利影响。在这项研究中,研究了 F 对豆根瘤菌的毒性,作为制定新西兰农业土壤 F 指导值的第一步。进行了基于瓶的实验以检查 F-离子对根瘤菌白三叶草 (Trifolium repens L. ) 通过观察结节形态和生长共生。结果表明,对于根瘤菌对豆类根瘤菌的 F- 毒性导致 10% 的根瘤菌呼吸抑制 (IC10) 的 F- 浓度 >100 mg F-L-1。当根瘤菌暴露于 500 和 1000 mg L-1 的 F 浓度时,会发生显着的形态变化。光显微照片和透射电子显微照片均证实豆根瘤菌-白三叶草相互作用不受 F-浓度 >100 mg L-1 的影响。本研究中确定的豆根瘤菌的有毒 F- 浓度比“正常条件”下新西兰农业土壤中的 F- 浓度高几个数量级。似乎没有迹象表明土壤 F 对豆根瘤菌有迫在眉睫的风险。结果表明,对于根瘤菌对豆类根瘤菌的 F- 毒性导致 10% 的根瘤菌呼吸抑制 (IC10) 的 F- 浓度 >100 mg F-L-1。当根瘤菌暴露于 500 和 1000 mg L-1 的 F 浓度时,会发生显着的形态变化。光显微照片和透射电子显微照片均证实豆根瘤菌-白三叶草相互作用不受 F-浓度 >100 mg L-1 的影响。本研究中确定的豆根瘤菌的有毒 F- 浓度比“正常条件”下新西兰农业土壤中的 F- 浓度高几个数量级。似乎没有迹象表明土壤 F 对豆根瘤菌有迫在眉睫的风险。结果表明,对于根瘤菌对豆类根瘤菌的 F- 毒性导致 10% 的根瘤菌呼吸抑制 (IC10) 的 F- 浓度 >100 mg F-L-1。当根瘤菌暴露于 500 和 1000 mg L-1 的 F 浓度时,会发生显着的形态变化。光显微照片和透射电子显微照片均证实豆根瘤菌-白三叶草相互作用不受 F-浓度 >100 mg L-1 的影响。本研究中确定的豆根瘤菌的有毒 F- 浓度比“正常条件”下新西兰农业土壤中的 F- 浓度高几个数量级。似乎没有迹象表明土壤 F 对豆根瘤菌有迫在眉睫的风险。
更新日期:2020-06-11
down
wechat
bug