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LncRNA TDRG1 promotes the proliferation, migration, and invasion of cervical cancer cells by sponging miR-214-5p to target SOX4
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-02-27 , DOI: 10.1080/10799893.2020.1731537
Meijun Guo 1 , Beibei Lin 1 , Guoping Li 2 , Jun Lin 1 , Xiuxiu Jiang 1
Affiliation  

Abstract The pathogenesis of cervical cancer (CC) at molecular level has attracted much research attention. The current study aimed to explore the effects of LncRNA TDRG1 on cellular process in CC cells and its molecular mechanism. Expressions of TDRG1 and miR-214-5p in CC and normal tissues and CC cells were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of TDRG1, miR-214-5p, and SOX4 on cell proliferation, migration, invasion, and EMT process of CC cells were detected by Cell Counting Kit-8 (CCK-8), colony formation, wound-healing, Transwell, and Western blot assays, respectively. StarBase and Targetscan7.2 were used to predict the target genes of TDRG1 and miR-214-5p, and the predictions were verified by dual-luciferase reporter assay. The expression of SOX4 in CC and normal tissues, and CC cells transfected with siTDRG1 or miR-214-5p inhibitor was determined by qRT-PCR. The results showed that expression of TDRG1 was up-regulated, while that of miR-214-5p was down-regulated in CC. The target genes of TDRG1 and miR-214-5p were verified to be miR-214-5p and SOX4, respectively. Knocking down TDRG1 expression could inhibit cell proliferation, colony, migration, and invasion abilities, and EMT process, whereas the inhibition of miR-214-5p expression partially reversed such results. Moreover, high SOX4 expression was observed in CC tissues, and down-regulating TDRG1 expression reduced the SOX4 expression while down-regulating miR-214-5p expression alleviated such an inhibition. In conclusion, TDRG1 acts as cancer promoter in CC through promoting cell proliferation, migration, invasion, and EMT process to modulate SOX4 expression through adsorbing miR-214-5p.

中文翻译:

LncRNA TDRG1通过海绵miR-214-5p靶向SOX4促进宫颈癌细胞增殖、迁移和侵袭

摘要 宫颈癌(CC)的分子水平发病机制引起了广泛的研究关注。本研究旨在探讨LncRNA TDRG1对CC细胞细胞过程的影响及其分子机制。通过定量实时聚合酶链反应 (qRT-PCR) 测量 TDRG1 和 miR-214-5p 在 CC 和正常组织和 CC 细胞中的表达。Cell Counting Kit-8 (CCK-8)检测TDRG1、miR-214-5p、SOX4对CC细胞增殖、迁移、侵袭和EMT过程的影响、集落形成、伤口愈合、Transwell、和蛋白质印迹分析,分别。StarBase和Targetscan7.2用于预测TDRG1和miR-214-5p的靶基因,并通过双荧光素酶报告基因检测验证预测。SOX4在CC和正常组织中的表达,通过qRT-PCR测定转染siTDRG1或miR-214-5p抑制剂的CC细胞。结果表明TDRG1的表达上调,而miR-214-5p的表达在CC中下调。TDRG1和miR-214-5p的靶基因经验证分别为miR-214-5p和SOX4。抑制 TDRG1 表达可以抑制细胞增殖、集落、迁移和侵袭能力以及 EMT 过程,而抑制 miR-214-5p 表达可以部分逆转这种结果。此外,在 CC 组织中观察到高 SOX4 表达,下调 TDRG1 表达降低了 SOX4 表达,而下调 miR-214-5p 表达减轻了这种抑制。总之,TDRG1通过促进细胞增殖、迁移、侵袭、
更新日期:2020-02-27
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