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Quantitative and qualitative analysis of integrin subtype expression in melanocytes and melanoma cells
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-02-17 , DOI: 10.1080/10799893.2020.1727923
Verena Kopatz 1 , Edgar Selzer 1
Affiliation  

Abstract Objectives: Changes in the integrin expression pattern have been associated with the malignant transformation of melanocytes suggesting that integrins may be potential biomarkers as well as molecular targets for individualized therapy. Since there is a lack of comprehensive qualitative and quantitative expression data, we characterized the integrin expression profile in normal and malignant human cells of the melanocytic lineage. Methods: Seven melanoma cell lines as well as normal human melanocytes were investigated in western blots including recombinant integrin subunits for quantification. Results: Expression patterns were heterogeneous. In melanoma, overexpression of α4, α6, αL, β5, and β6 was found. Integrins α7, α9, and β4 were overexpressed in a subset of the melanoma cell lines. Overexpression was defined as a lack of expression in melanocytes but expression in more than half (4) of the melanoma lines. 1.9 to 6.7 × 106 integrin molecules (about 0.3% of total cellular protein) were estimated to be expressed per cell. Expression of integrin αE at the protein level was found in melanoma and melanocytes, to the best of our knowledge, for the first time. Integrins αM and β2 were not detected. Conclusion: Integrins α4, α6, αL, β5, and β6 appear to be overexpressed in melanoma cells. These subunits may serve as biomarkers and/or therapeutic targets.

中文翻译:

黑色素细胞和黑色素瘤细胞中整合素亚型表达的定量和定性分析

摘要目的:整合素表达模式的变化与黑色素细胞的恶性转化有关,表明整合素可能是潜在的生物标志物以及个体化治疗的分子靶点。由于缺乏全面的定性和定量表达数据,我们对黑素细胞谱系的正常和恶性人类细胞中的整联蛋白表达谱进行了表征。方法:在蛋白质印迹中研究了七种黑色素瘤细胞系以及正常人黑色素细胞,包括用于定量的重组整合素亚基。结果:表达模式是异质的。在黑色素瘤中,发现了 α4、α6、αL、β5 和 β6 的过度表达。整合素 α7、α9 和 β4 在黑色素瘤细胞系的一个子集中过度表达。过度表达被定义为在黑素细胞中缺乏表达,但在超过一半 (4) 的黑素瘤系中表达。估计每个细胞表达 1.9 到 6.7 × 106 个整联蛋白分子(约占细胞总蛋白的 0.3%)。据我们所知,首次在黑色素瘤和黑色素细胞中发现了整合素 αE 在蛋白质水平上的表达。未检测到整联蛋白 αM 和 β2。结论:整合素 α4、α6、αL、β5 和 β6 似乎在黑色素瘤细胞中过度表达。这些亚基可以作为生物标志物和/或治疗靶点。据我们所知,这是第一次。未检测到整联蛋白 αM 和 β2。结论:整合素 α4、α6、αL、β5 和 β6 似乎在黑色素瘤细胞中过度表达。这些亚基可以作为生物标志物和/或治疗靶点。据我们所知,这是第一次。未检测到整联蛋白 αM 和 β2。结论:整合素 α4、α6、αL、β5 和 β6 似乎在黑色素瘤细胞中过度表达。这些亚基可以作为生物标志物和/或治疗靶点。
更新日期:2020-02-17
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