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Cell wall/vacuolar inhibitor of fructosidase 1 regulates ABA response and salt tolerance in Arabidopsis
Plant Signaling & Behavior ( IF 2.9 ) Pub Date : 2020-03-26 , DOI: 10.1080/15592324.2020.1744293
Wenting Yang 1 , Siyu Chen 1 , Yuxin Cheng 1 , Na Zhang 1 , Yanxing Ma 1 , Wei Wang 1 , Hainan Tian 1 , Yingying Li 1 , Saddam Hussain 1 , Shucai Wang 1, 2
Affiliation  

ABSTRACT ABA regulates abiotic stress tolerance in plants via activating/repressing gene expression. However, the functions of many ABA response genes remained unknown. C/VIFs are proteinaceous inhibitors of the CWI and VI invertases. We report here the involvement of C/VIF1 in regulating ABA response and salt tolerance in Arabidopsis. We found that the expression level of C/VIF1 was increased in response to ABA treatment. By using CRISPR/Cas9 gene editing, we generated transgene-free c/vif1 mutants. We also generated C/VIF1 overexpression plants by expressing C/VIF1 under the control of the 35S promoter. We examined ABA response of the 35S:C/VIF1 transgenic plants and the c/vif1 mutants by using seed germination and seedling greening assays, and found that the 35S:C/VIF1 transgenic plants showed an enhanced sensitivity to ABA treatment in both assays. On the other hand, the c/vif1 mutants showed slight enhanced tolerance to ABA only at the early stage of germination. We also found that salt tolerance was reduced in the 35S:C/VIF1 transgenic plants in seed germination assays, but slightly increased in the c/vif1 mutants. Taken together, our results suggest that C/VIF1 is an ABA response gene, and C/VIF1 is involved in the regulation of ABA response and salt tolerance in Arabidopsis.

中文翻译:

果糖苷酶 1 的细胞壁/液泡抑制剂调节拟南芥中的 ABA 反应和耐盐性

摘要 ABA 通过激活/抑制基因表达调节植物的非生物胁迫耐受性。然而,许多 ABA 反应基因的功能仍然未知。C/VIF 是 CWI 和 VI 转化酶的蛋白质抑制剂。我们在此报告 C/VIF1 参与调节拟南芥中的 ABA 反应和耐盐性。我们发现 C/VIF1 的表达水平响应 ABA 处理而增加。通过使用 CRISPR/Cas9 基因编辑,我们生成了无转基因 c/vif1 突变体。我们还通过在 35S 启动子的控制下表达 C/VIF1 来生成 C/VIF1 过表达植物。我们通过种子萌发和幼苗绿化试验检测了 35S:C/VIF1 转基因植物和 c/vif1 突变体的 ABA 反应,发现 35S:C/VIF1 转基因植物在两种测定中都对 ABA 处理表现出增强的敏感性。另一方面,c/vif1 突变体仅在萌发早期对 ABA 的耐受性略有增强。我们还发现在种子萌发试验中 35S:C/VIF1 转基因植物的耐盐性降低,但在 c/vif1 突变体中略有增加。总之,我们的结果表明 C/VIF1 是一种 ABA 反应基因,C/VIF1 参与了拟南芥 ABA 反应和耐盐性的调节。
更新日期:2020-03-26
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