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Expression of rice siR109944 in Arabidopsis affects plant immunity to multiple fungal pathogens
Plant Signaling & Behavior ( IF 2.9 ) Pub Date : 2020-03-23 , DOI: 10.1080/15592324.2020.1744347
Lulu Qiao 1, 2 , Hongwei Zhao 1, 2 , Hailing Jin 3 , Dongdong Niu 1, 2
Affiliation  

ABSTRACT Plant small RNA (sRNA)-mediated gene expression has a conserved role in regulating plant growth, development, and immunity. Heterologous expression of sRNA contributes to determining whether the function of sRNA is conservative or independent. We recently characterized the Tourist-miniature inverted-repeat transposable element (MITE)-derived siR109944 had a conserved function that enhanced susceptibility to Rhizoctonia solani infection by affecting auxin homeostasis in rice and Arabidopsis. To ascertain whether the function of rice siR109944 has a broad-spectrum immunity in Arabidopsis, we infected Arabidopsis with a variety of fungal pathogens. Overexpression of siR109944 in Arabidopsis increased susceptibility to Botrytis cinerea, Sclerotinia sclerotium, and Verticillium dahliae infection. Further studies found that Arabidopsis auxin-related miRNAs were suppressed in siR109944 OE. Our results demonstrated that overexpression of rice siR109944 in Arabidopsis affected immune responses to multiple pathogens by inhibiting auxin-related miRNA expression in planta.

中文翻译:

水稻 siR109944 在拟南芥中的表达影响植物对多种真菌病原体的免疫

摘要 植物小 RNA (sRNA) 介导的基因表达在调节植物生长、发育和免疫方面具有保守作用。sRNA 的异源表达有助于确定 sRNA 的功能是保守的还是独立的。我们最近表征了旅游微型反向重复转座因子 (MITE) 衍生的 siR109944 具有保守的功能,通过影响水稻和拟南芥中的生长素稳态来增强对立枯丝核菌感染的易感性。为了确定水稻 siR109944 的功能是否在拟南芥中具有广谱免疫,我们用多种真菌病原体感染拟南芥。siR109944 在拟南芥中的过表达增加了对灰葡萄孢、菌核和大丽轮枝菌感染的易感性。进一步的研究发现,拟南芥生长素相关的 miRNA 在 siR109944 OE 中受到抑制。我们的结果表明,水稻 siR109944 在拟南芥中的过表达通过抑制植物中生长素相关 miRNA 的表达来影响对多种病原体的免疫反应。
更新日期:2020-03-23
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