In this study, three TaEDS1 genes from wheat (Triticum aestivum) were heterologously expressed in Arabidopsis, and their functions under salt stress were analyzed. Promoter analysis indicated that the TaEDS1-5D promoter sequence included a variety of stress response elements and the TaEDS1 transcript level was particularly enhanced after salt treatment which suggests that the TaEDS1 genes might play a role in salt stress. Physiological analyses indicated that in transgenic plants, chlorophyll and proline contents were elevated in comparison to eds1 mutants, and a smaller decrease in the K⁺/Na⁺ ratio than the eds1 mutants. Further analysis showed that the transgenic lines underwent larger elevations in protein content, superoxide dismutase, and catalase activity, and a smaller increase in the malondialdehyde content than the eds1 mutants. This indicated that transgenic lines displayed a better antioxidant response to reduce the reactive oxygen species content than eds1 lines. Together, the results showed that TaEDS1 genes may participate in the process of salt tolerance.

在这项研究中，小麦（Triticum aestivum）的三个TaEDS1基因在拟南芥中异源表达，并分析了它们在盐胁迫下的功能。启动子分析表明，TaEDS1-5D启动子序列包括多种应激反应元件，盐处理后TaEDS1转录水平特别提高，这表明TaEDS1基因可能在盐胁迫中起作用。生理分析表明，与eds1突变体相比，转基因植物中的叶绿素和脯氨酸含量升高，而K ⁺ / Na ⁺降低幅度较小。比eds1突变体。进一步的分析表明，与eds1突变体相比，转基因品系的蛋白质含量，超氧化物歧化酶和过氧化氢酶活性升高幅度更大，丙二醛含量升高幅度较小。这表明转基因品系显示出比eds1品系更好的抗氧化反应，以减少活性氧含量。总之，结果表明TaEDS1基因可能参与了耐盐性过程。