The NAC (NAM-ATAF1/2-CUC) transcription factors (TFs) regulate numerous biological processes, such as growth, development, and stress responses. The yield and quality of Bambusa emeiensis, an economically important bamboo, decrease under stress caused by insect herbivores, such as Cyrtotrachelus buqueti. In the present study, 33 BeNACs, including 4 membrane-associated TFs, were identified in B. emeiensis transcriptome. Phylogenetic analysis revealed that BeNACs and their Arabidopsis thaliana counterparts clustered into 4 major groups, which were subdivided into 17 subgroups. Conserved motif and phylogenetic analyses revealed that BeNACs with close evolutionary relationships contained highly similar motifs. The N-terminal regions of BeNACs had NAC domains. In addition, the C-termini and transmembrane domains of four BeNACs contained transmembrane motifs. Transcriptome analysis revealed that majority of BeNACs were highly expressed under herbivory. The expression levels of eight BeNACs, including predicted stress-related and membrane-bound BeNACs, in bamboo shoots, shells, trichomes, and leaves and under two treatments (fed and unfed) were assessed through quantitative real-time polymerase chain reaction. Several BeNACs (BeNAC4, 10, 19, and 24) were considered as closely related to responses to herbivore. This study lays a foundation for future study of BeNACs’ functions in bamboo development and stress response.

NAC（NAM-ATAF1 / 2-CUC）转录因子（TFs）调节许多生物学过程，例如生长，发育和应激反应。经济上重要的竹子峨眉草（Bambusa emeiensis）的产量和品质在昆虫食草动物（如Cyrtotrachelus buqueti）引起的胁迫下会降低。在本研究中，在峨眉杆菌转录组中鉴定出33个BeNAC，包括4个与膜相关的TF 。系统发育分析表明，BeNAC及其拟南芥对应对象分为4个主要组，再分为17个子组。保守的基序和系统发育分析表明，具有密切进化关系的BeNAC包含高度相似的基序。BeNAC的N端区域具有NAC域。此外，四个BeNAC的C末端和跨膜域包含跨膜基序。转录组分析显示大多数BeNAC在草食性下均高度表达。通过定量实时聚合酶链反应评估了八种BeNAC在竹笋，贝壳，毛状体和叶片中的表达水平，包括预测的与胁迫相关的和与膜结合的BeNAC的表达，以及两种处理（喂食和不喂食）下的表达。几种BeNAC（BeNAC4、10、19和24）被认为与对草食动物的反应密切相关。