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Bioluminescence emissions from the Indian winter species of firefly Diaphanes sp.
Journal of Biosciences ( IF 2.9 ) Pub Date : 2020-04-22 , DOI: 10.1007/s12038-020-00033-6
Mana Mohan Rabha , Upamanyu Sharma , Anurup Gohain Barua

Numerous studies have been carried out on different aspects of the light from summer-active fireflies. Characteristics of this light have led to very interesting conclusions on the chemiluminescence reaction as well as on the nature of the light from live fireflies. Here we present a first report on bioluminescence emissions from a newly found winter-active Indian species of firefly Diaphanes sp. The steady-state emission spectrum from this species comes out to be apparently similar to those from the other two Indian summer species, Luciola praeusta and Asymmetricata circumdata: asymmetric in nature with a little bit of change in the position of the peak wavelength and in the width of the full width at half maximum. An increase in temperature to approximately 28°C causes a red-shift in the peak wavelength, which probably indicates denaturation of the enzyme luciferase in the live, flashing condition. Emissions in the time domain reveal that the light is never completely off – it decreases in intensity to a low value, sometimes very close to zero, and then increases – a characteristic unheard-of till date. Flash durations are considerably longer than those from the two Indian summer species; those become shorter at about 28°C and increase to noticeably larger values at higher temperatures.

中文翻译:

印度冬季萤火虫 Diaphanes sp. 的生物发光发射。

已经对夏季活跃的萤火虫发出的光的不同方面进行了大量研究。这种光的特性导致了关于化学发光反应以及来自活萤火虫的光的性质的非常有趣的结论。在这里,我们提出了关于新发现的冬季活跃的印度萤火虫 Diaphanes sp 的生物发光发射的第一份报告。该物种的稳态发射光谱与其他两种印度夏季物种 Luciola praeusta 和 Asymmetricata circledata 的稳态发射光谱明显相似:本质上是不对称的,峰值波长的位置和波长略有变化。半高全宽的宽度。温度升高到大约 28°C 会导致峰值波长发生红移,这可能表明荧光素酶在活的、闪烁的条件下发生了变性​​。时域中的发射表明光永远不会完全关闭——它的强度降低到一个低值,有时非常接近于零,然后增加——这是迄今为止闻所未闻的特征。闪光持续时间比这两种印度夏季物种长得多;这些在大约 28°C 时变得更短,并在更高的温度下增加到明显更大的值。
更新日期:2020-04-22
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