Tissue analysis by Fourier transform infrared (FTIR) imaging can determine the biodistribution of molecules, without pre-analytical modification. We aimed to study the infrared spectroscopic changes of α-helical proteins at post-traumatic epileptic (PTE) foci by FTIR. FITR mapping was applied to detect α-helical proteins in rat brain tissue samples with post-traumatic epilepsy. Histological examination of brain sections showed that the rat model of PTE was successfully established. At the PTE foci, high α-helical absorption regions were evident, where the color difference and absorption were significantly different from those in the low-absorption regions. This provided a distinctive and characteristic pattern at the site of lesions. The use of FTIR imaging means that it is possible to measure the molecular structural changes resulting from PTE pathologies in tissues, providing a novel adjunct to conventional pathological diagnostic techniques.

中文翻译：

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通过傅里叶变换红外测绘监测创伤后癫痫灶的α-螺旋蛋白吸收

通过傅里叶变换红外 (FTIR) 成像进行的组织分析可以确定分子的生物分布，而无需进行分析前的修改。我们的目的是通过 FTIR 研究 α-螺旋蛋白在创伤后癫痫 (PTE) 病灶的红外光谱变化。FITR 映射用于检测大鼠创伤后癫痫脑组织样本中的α-螺旋蛋白。脑切片组织学检查表明，PTE大鼠模型建立成功。在 PTE 焦点处，明显存在高 α-螺旋吸收区，其中色差和吸收与低吸收区显着不同。这在病变部位提供了独特的特征模式。