In this study, 2n gametes were induced in the rubber tree RRIM 600 by high-temperature exposure and used for the production of triploid offspring. A relationship between megasporogenesis and the external morphology of male and female flower buds was established to guide the treatments. The relationship between megasporogenesis and microsporogenesis of adjacent male flower buds was also estimated. The developmental stage of megasporogenesis was estimated indirectly based on these relationships. Finally, 5 open-pollinated triploid offspring were obtained; the mean efficiency of triploid production was approximately 1.2%. The induction efficiency was approximately 9.1% when meiotic stages of megasporogenesis from the diplotene stage to the diakinesis stage were exposed to a high temperature of 44 °C for 4 h. Simple sequence repeat (SSR) markers revealed that the 2n gametes were derived from the RRIM 600 female parent. This study was the first to induce 2n female gametes in rubber trees and will serve as the basis for the germplasm creation of triploids and breeding of new varieties of rubber trees.

在这项研究中，通过高温暴露在橡胶树RRIM 600中诱导了2n个配子，并用于生产三倍体后代。建立大孢子发生与雄性和雌性花蕾的外部形态之间的关系以指导治疗。还估计了相邻雄花芽的大孢子发生与小孢子发生之间的关系。基于这些关系间接估计了大孢子发生的发育阶段。最后，获得了5个开放授粉的三倍体后代。三倍体生产的平均效率约为1.2％。当将大麦芽孢形成的减数分裂阶段（从二倍体阶段到重金属化阶段）暴露于44°C的高温4 h时，诱导效率约为9.1％。简单序列重复（SSR）标记显示2n配子来自RRIM 600母本。这项研究是第一个在橡胶树中诱导2n雌配子的研究，并将作为三倍体种质创造和橡胶树新品种育种的基础。