Tobacco (Nicotiana tabacum L.) is an important agronomic crop and model system for studies of plant-pathogen interactions. Black shank, caused by Phytophthora nicotianae, is an important disease affecting tobacco production worldwide. In this study, a mapping population of 177 F_7:8-9 recombinant inbred lines was generated from a cross between the highly resistant cultivar ‘Yunyan 85’ and a susceptible line ‘Dabaijin 599’. A high-density genetic linkage map containing 7734 single-nucleotide polymorphic markers based on restriction site-associated DNA tag sequencing technology was used to finely map quantitative trait loci (QTL) for resistance to P. nicotianae. A total of 10 QTLs were detected as being associated with resistance to P. nicotianae across multiple environments, and two major QTL qBS7 and qBS14 were repeatedly identified under all five environments. They explained 16.48-62.20% and 3.94-11.29% of the phenotypic variance with high LOD score, respectively. One hundred thirty-eight candidate genes were identified for two major QTLs qBS7 and qBS14, and annotation analysis showing that several predicted genes encoded proteins associated with plant defense response to pathogens. This high-density single-nucleotide polymorphic genetic linkage map of flue-cured tobacco based on restriction site-associated DNA sequencing was useful in the QTL finely mapping of resistance to P. nicotianae. This study increases our understanding of the genetics of resistance to P. nicotianae and aids in marker-assisted selection.

烟草（Nicotiana tabacum L.）是研究植物与病原体相互作用的重要农作物和模型系统。由烟草疫霉（Phytophthora nicotianae）引起的黑胫病是影响全世界烟草生产的重要疾病。在这项研究中，从高抗性品种“云烟85”和易感品系“大白金599”之间的杂交产生了177 F _7：8-9重组自交系的作图群体。高密度遗传连锁图谱包含基于限制酶切位点相关的DNA标签测序技术的7734个单核苷酸多态性标记，用于精细绘制定量特征基因位点（QTL），以鉴定对烟草的抗性。总共检测到10个QTL与耐药性相关跨多个环境的烟草假单胞菌，并且在所有五个环境中都反复鉴定出两个主要的QTL qBS7和qBS14。他们分别解释了具有高LOD评分的表型变异的16.48-62.20％和3.94-11.29％。鉴定了两个主要QTL qBS7和qBS14的138个候选基因，注释分析显示，几个预测基因编码与植物对病原体防御反应相关的蛋白质。这种基于限制性位点相关DNA测序的烤烟高密度单核苷酸多态性遗传连锁图谱可用于QTL精细绘制对烟草假单胞菌的抗性。这项研究增加了我们对烟草假单胞菌抗性的遗传学的了解，并有助于标记辅助选择。