Crown rot of wheat and barley is a serious disease caused by the fungus Fusarium pseudograminearum, a stubble-borne pathogen common in no-till farming systems in water-limited grain growing regions. Marker assisted recurrent selection (MARS) was used to more effectively pyramid minor genes controlling plant response to crown rot disease. Two populations comprising 475 genotypes were phenotyped for adult plant resistance and genotyped using a 9K SNP array. A genome-wide association study (GWAS) was used to estimate quantitative trait locus (QTL) effects as more than two parents were recombined in each population. A total of 23 significant and unique marker-trait associations (MTAs) were identified in both populations. A Kompetitive allele-specific PCR (KASP) assay was developed and used to genotype the progeny and parents of each recombination cycle. Following two cycles of recombination among progeny, up to 22 markers of a possible 23 were recombined. Two hundred ninety-seven double haploid-derived lines representing both cycles of recombination, the original parents, parents selected from the base population and local cultivars were evaluated for crown rot resistance in 2016 and 2017 in the field under crown rot inoculation pressure. A significant and positive response to each cycle of recurrent selection was observed. MARS was an effective breeding strategy for combining many QTLs of small effect for crown rot resistance in wheat.

小麦和大麦的冠腐病是由真菌镰刀镰刀菌引起的严重疾病是在缺水的谷物种植区的免耕耕作系统中常见的由胡茬传播的病原体。标记辅助轮回选择（MARS）用于更有效地金字塔控制植物对冠腐病反应的次要基因。利用成年植物抗性对两个包含475个基因型的种群进行表型分析，并使用9K SNP阵列进行基因分型。全基因组关联研究（GWAS）用于估计数量性状基因座（QTL）的作用，因为每个群体中有两个以上的父母重组。在这两个人群中总共鉴定出23个重要且独特的标记-性状关联（MTA）。开发了竞争竞争性等位基因特异性PCR（KASP）分析，并用于对每个重组周期的后代和亲本进行基因分型。在子代之间进行了两个重组周期后，重组了可能的23种标记中的22种。在2016年和2017年在冠腐病接种压力下，对代表两个重组周期的279个双单倍体衍生品系，原始亲本，从基本种群中选出的亲本和当地品种进行了评估，以研究其冠腐病抗性。观察到对循环选择的每个循环的显着积极反应。MARS是一种有效的育种策略，可以结合许多对小麦的冠腐病抗性影响小的QTL。观察到对循环选择的每个循环的显着积极反应。MARS是一种有效的育种策略，可以结合许多对小麦的冠腐病抗性影响小的QTL。观察到对循环选择的每个循环的显着积极反应。MARS是一种有效的育种策略，可以结合许多对小麦的冠腐病抗性影响小的QTL。