Background

Mitochondrial DNA (mtDNA) copy number and deletion ratio may be asymmetrically determined using physiologic or pathologic conditions. Mitochondrial dysfunction is associated with an increased risk of developing metabolic syndrome (MetS).

Objective

Here, we investigated the association between mtDNA copy number and/or deletion ratio and the risk of developing MetS in a general population exposed to the pesticide. We examined 215 randomly sampled adults who were exposed to pesticide but did not present with MetS in a prospective cohort study. Both mtDNA copy number and deletion ratio were determined using quantitative real-time polymerase chain reaction.

Results

During the average 2.8-year follow-up period, 76 (35.3%) participants developed new-onset MetS. The numbers of mtDNA copies were significantly lower in participants with new-onset MetS than in those without MetS (112.15 ± 26.15 vs. 133.13 ± 44.44, p < 0.001), whereas the mtDNA deletion ratios were higher in participants with MetS than in those without MetS (10.59 ± 11.96 vs. 4.52 ± 6.74, p < 0.001). In the multivariate-adjusted models, the participants with a higher tertile of mtDNA copy number were 0.355 times more likely to develop MetS than those with the lowest tertile [odds ratio (OR) 0.355, 95% confidence interval (CI) 0.150–0.841, p = 0.004]. A higher mtDNA deletion ratio was significantly associated with the risk of developing MetS in populations exposed to pesticide (OR 5.062, 95% CI 1.164–22.004, p = 0.004).

Conclusion

A lower mtDNA copy number and higher deletion ratio were independent predictors for new-onset MetS in a general population exposed to the pesticide.

中文翻译：

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在接触农药的普通人群中，线粒体DNA含量和缺失率与代谢综合征相关

背景

线粒体DNA（mtDNA）的拷贝数和缺失率可以使用生理或病理条件非对称地确定。线粒体功能障碍与发生代谢综合征（MetS）的风险增加相关。

目的

在这里，我们调查了暴露于该农药的普通人群中mtDNA拷贝数和/或缺失率与发展MetS风险之间的关联。在一项前瞻性队列研究中，我们检查了215名随机抽样的成年人，这些成年人曾接触过农药但未出现MetS。使用定量实时聚合酶链反应确定mtDNA的拷贝数和缺失率。

结果

在平均2.8年的随访期内，有76名（35.3％）参与者患上了新发病例的MetS。有新发MetS的参与者的mtDNA拷贝数显着低于没有MetS的参与者（112.15±26.15 vs. 133.13±44.44，p <0.001），而有MetS的参与者的mtDNA缺失率更高。大都会（10.59±11.96 vs.4.52±6.74，p  <0.001）。在多变量调整模型中，具有较高三分位数mtDNA拷贝数的参与者发生MetS的可能性比具有最低三分位数[OR] 0.355、95％置信区间（CI）0.150-0.841的参与者高0.355倍。p = 0.004]。较高的mtDNA缺失率与接触农药的人群发生MetS的风险显着相关（OR 5.062，95％CI 1.164-22.004，p  = 0.004）。

结论

较低的mtDNA拷贝数和较高的缺失率是暴露于该农药的普通人群中新发MetS的独立预测因子。