Gentianella acuta (G. acuta), as a folk medicine, was used to treat heart disease by the Ewenki people in Inner Mongolia. However, the effect of G. acuta on acute myocardial infarction (AMI) is not clear. To explore the mechanisms of G. acuta on isoproterenol (ISO)-induced AMI, rats were administered G. acuta for 28 days, then injected intraperitoneally with ISO (85 mg/kg) on days 29 and 30. An electrocardiogram helped to evaluate the myocardial injury. Serum lactate dehydrogenase (LDH), creatinine kinase (CK) and aspartate aminotransferase (AST) levels were evaluated, and haematoxylin eosin, Masson's trichrome staining and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining were used to detect myocardial histological changes. Radioimmunoassay was used to measure serum tumour necrosis factor alpha (TNFα) and interleukin (IL)-6. An enzyme-linked immunosorbent assay kit was used to analyse serum galectin-3 (Gal-3) levels. Immunohistochemistry, Western blotting and reverse transcription polymerase chain reaction were used to examine relevant molecular events. The results revealed that pre-treatment with G. acuta decreased the elevation in the ST segment; reduced serum LDH, CK and AST levels; alleviated cardiac structure disorder; and reduced inflammatory infiltration, abnormal collagen deposition and cardiomyocyte apoptosis that were induced by ISO. Furthermore, pre-treatment with G. acuta inhibited serum Gal-3 levels and Gal-3 expression in heart tissue, and also impeded TLR4/MyD88/NF-кB signalling activation, which ultimately prevented the expression of inflammatory cytokines. The study indicated that pre-treatment with G. acuta protects against ISO-induced AMI, and the protective role may be related to inhibiting Gal-3/TLR4/MyD88/NF-кB inflammatory signalling.

中文翻译：

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Gentianella acuta 通过抑制半乳糖凝集素-3/TLR4/MyD88/NF-кB 炎症信号传导，预防异丙肾上腺素诱导的大鼠急性心肌梗死。

Gentianella acuta (G. acuta) 作为一种民间药物，被内蒙古鄂温克人用来治疗心脏病。然而，G. acuta 对急性心肌梗死 (AMI) 的影响尚不清楚。为了探索 G. acuta 对异丙肾上腺素 (ISO) 诱导的 AMI 的机制，大鼠服用 G. acuta 28 天，然后在第 29 天和第 30 天腹腔注射 ISO (85 mg/kg)。心电图有助于评估心肌损伤。评估血清乳酸脱氢酶 (LDH)、肌酐激酶 (CK) 和天冬氨酸转氨酶 (AST) 水平，并使用苏木精伊红、马森三色染色和末端脱氧核苷酸转移酶 dUTP 缺口末端标记 (TUNEL) 染色检测心肌组织学变化。采用放射免疫法测定血清肿瘤坏死因子α（TNFα）和白细胞介素（IL）-6。使用酶联免疫吸附测定试剂盒分析血清半乳糖凝集素-3 (Gal-3) 水平。免疫组织化学、蛋白质印迹和逆转录聚合酶链反应用于检查相关分子事件。结果表明，G. acuta 预处理降低了 ST 段的抬高；降低血清 LDH、CK 和 AST 水平；减轻心脏结构紊乱；并减少 ISO 诱导的炎症浸润、异常胶原沉积和心肌细胞凋亡。此外，G. acuta 预处理抑制了心脏组织中血清 Gal-3 水平和 Gal-3 表达，也阻碍了 TLR4/MyD88/NF-кB 信号激活，这最终阻止了炎性细胞因子的表达。研究表明，G. acuta 预处理可预防 ISO 诱导的 AMI，其保护作用可能与抑制 Gal-3/TLR4/MyD88/NF-кB 炎症信号传导有关。