Microbial bioconversion of a given substrate is considered an efficient and eco-friendly tool for value-added industrial compound generation from natural products. Among natural products, unsaturated fatty acids have been used as substrates to produce various functional hydroxy fatty acids. In this study, we report the production of 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid by a new strain, Pseudomonas aeruginosa KNU-2B. KNU-2B efficiently produced DHD from palmitoleic acid and required a strong alkaline condition for maximum DHD production. The maximum DHD amount produced under pH 10.0 and 48-h incubation at 27 °C and 150 rpm was 219.5 mg/100 mL culture. Other important nutritional factors were also investigated to obtain optimum DHD production.

中文翻译：

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铜绿假单胞菌KNU-2B从棕榈油酸生产7,10-二羟基-8(E)-十六碳烯酸优选强碱性条件

给定底物的微生物生物转化被认为是一种有效且环保的工具，可用于从天然产物中生成增值工业化合物。在天然产物中，不饱和脂肪酸已被用作生产各种功能性羟基脂肪酸的底物。在这项研究中，我们报告了新菌株铜绿假单胞菌 KNU-2B 从棕榈油酸生产 7,10-二羟基-8(E)-十六碳烯酸 (DHD)。KNU-2B 从棕榈油酸有效地生产 DHD，并且需要强碱性条件才能最大程度地生产 DHD。在 pH 10.0 和 27 °C 和 150 rpm 培养 48 小时下产生的最大 DHD 量为 219.5 mg/100 mL 培养物。还研究了其他重要的营养因素以获得最佳的 DHD 产量。