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Isolation and characterization of 2-butoxyethanol degrading bacterial strains.
Biodegradation ( IF 3.6 ) Pub Date : 2020-04-30 , DOI: 10.1007/s10532-020-09900-3
Christine Woiski 1 , Daniel Dobslaw 1 , Karl-Heinrich Engesser 1
Affiliation  

A total of 11 bacterial strains capable of completely degrading 2-butoxyethanol (2-BE) were isolated from forest soil, a biotrickling filter, a bioscrubber, and activated sludge, and identified by 16S rRNA gene sequence analysis. Eight of these strains belong to the genus Pseudomonas; the remaining three strains are Hydrogenophaga pseudoflava BOE3, Gordonia terrae BOE5, and Cupriavidus oxalaticus BOE300. In addition to 2-BE, all isolated strains were able to grow on 2-ethoxyethanol and 2-propoxyethanol, ethanol, n-hexanol, ethyl acetate, 2-butoxyacetic acid (2-BAA), glyoxylic acid, and n-butanol. Apart from the only gram-positive strain isolated, BOE5, none of the strains were able to grow on the nonpolar ethers diethyl ether, di-n-butyl ether, n-butyl vinyl ether, and dibenzyl ether, as well as on 1-butoxy-2-propanol. Strains H. pseudoflava BOE3 and two of the isolated pseudomonads, Pseudomonas putida BOE100 and P. vancouverensis BOE200, were studied in more detail. The maximum growth rates of strains BOE3, BOE100, and BOE200 at 30 °C were 0.204 h−1 at 4 mM, 0.645 h−1 at 5 mM, and 0.395 h−1 at 6 mM 2-BE, respectively. 2-BAA, n-butanol, and butanoic acid were detected as potential metabolites during the degradation of 2-BE. These findings indicate that the degradation of 2-BE by the isolated gram-negative strains proceeds via oxidation to 2-BAA with subsequent cleavage of the ether bond yielding glyoxylate and n-butanol. Since Gordonia terrae BOE5 was the only strain able to degrade nonpolar ethers like diethyl ether, the degradation pathway of 2-BE may be different for this strain.

中文翻译:

降解2-丁氧基乙醇的细菌菌株的分离和鉴定。

从森林土壤,生物滴滤池,生物洗涤塔和活性污泥中分离出总共11种能够完全降解2-丁氧基乙醇(2-BE)的细菌菌株,并通过16S rRNA基因序列分析进行了鉴定。这些菌株中有8个属于假单胞菌属; 剩余的三个菌株Hydrogenophaga pseudoflava BOE3,大头terrae BOE5,和贪铜oxalaticus BOE300。除了2-BE,所有分离的菌株能够在2-乙氧基乙醇和2-丙氧基乙醇,乙醇,生长Ñ己醇,乙酸乙酯,乙酸2-丁氧基乙酸(2-BAA),二羟乙酸,和Ñ-丁醇。除了分离出的唯一革兰氏阳性菌株BOE5外,所有菌株都无法在非极性醚二乙醚,二丁基醚,丁基乙烯基醚和二苄基醚以及1-苯基上生长。丁氧基-2-丙醇。菌株H. pseudoflava BOE3和两个分离的假单胞菌,恶臭假单胞菌BOE100和P. vancouverensis BOE200进行了更详细的研究。BOE3,BOE100和BOE200菌株在30°C时的最大生长速率在4 mM时为0.204 h -1,在5 mM时为0.645 h -1,在6 mM 2-BE中为0.395 h -1。2-BAA,n-2-丁醇和丁酸被认为是2-BE降解过程中的潜在代谢产物。这些发现表明,分离的革兰氏阴性菌株对2-BE的降解通过氧化成2-BAA进行,随后醚键的裂解产生乙醛酸酯和丁醇。由于Gordonia terra BOE5是唯一能够降解非极性醚(如乙醚)的菌株,因此2-BE的降解途径可能与此菌株不同。
更新日期:2020-04-30
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