Soybean [Glycine max (L.) Merr.] is one of the most complete food crops and has fed people and livestock since its domestication. Soybean androgenesis has been a challenging process yet to be advanced significantly. There have been minor advances, e.g., reports of a 2% induction rate in anther culture, formation of roots, rare shoots, but few plantlets and these did not survive to maturity. This research attempted to identify pyramidal stressors (the combination of temperature shock and incubation conditions) for soybean androgenesis using anther cultures. The results across genotypes Jack, Thorne, Williams 82 and IAS-5 indicated that androgenesis can be stimulated up to 9 to 12% induction frequency by the use of 10°C day/8°C night for 3 d then 4°C overnight dark pretreatment of donor plants, a series of incubation temperatures from 11°C to 18°C to 25°C, and nitrogen starvation medium. The adaptation of the anther culture protocol for isolated microspore cultures of IAS-5 and Embrapa-1 resulted in more than 90% of culture replicates (ave. 4 × 10⁵ microspores mL⁻¹, 500 μL per replicate) exhibiting sustained cell divisions with complex multicellular structures, including formation of one or more putative embryos. These results are a promising step towards the use of microspore cultures for soybean androgenesis, and a possible route to doubled haploid breeding for soybean.

大豆[ Glycine max（L.）Merr。]是最完整的粮食作物之一，自驯化以来已为人类和牲畜提供了饲料。大豆雄激素生成是一个具有挑战性的过程，尚未得到显着发展。有一些小进步，例如，据报道在花药培养中诱导率为2％，形成根，稀有芽，但幼苗很少，这些不能存活到成熟。这项研究试图使用花药培养物确定用于大豆雄激素生成的金字塔形压力源（温度冲击和孵育条件的组合）。杰克，索恩，威廉姆斯82和IAS-5基因型的结果表明，通过在白天10°C / 8°C夜间使用3 d，然后在黑暗中4°C过夜，可以将雄激素生成的刺激频率提高到9-12％预处理供体植物，一系列培养温度从11°C到18°C到25°C，以及氮饥饿培养基。花药培养方案对IAS-5和Embrapa-1的分离小孢子培养物的适应性导致90％以上的培养物重复（平均4×10 ⁵微孢子（mL ^-1，每个重复500μL）表现出具有复杂多细胞结构的持续细胞分裂，包括一个或多个推定胚胎的形成。这些结果是朝着将小孢子培养物用于大豆雄激素生成迈出的有希望的一步，也是大豆单倍体育种翻倍的可能途径。