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Comparison of oil emulsion, mannosylated chitosan, and Bacillus vector adjuvants for vaccination against influenza in chickens
Journal of Applied Poultry Research ( IF 1.9 ) Pub Date : 2020-04-30 , DOI: 10.1016/j.japr.2020.04.003
Christine N. Vuong , Vivek A. Kuttappan , Olivia B. Faulkner , Luc R. Berghman , Amanda D. Wolfenden , Guillermo Tellez-Isaias , Melina Jonas , Darrell R. Kapczynski , Billy M. Hargis , Lisa R. Bielke

Vaccination against influenza continues to be the primary method of control in many countries, but there is still a strong reliance on autogenous vaccines adjuvated with mineral oil emulsions known to induce overly high local inflammatory responses and lesions at the site of injection. The studies presented herein investigate various combinations of mannosylated chitosan adjuvant (MCA) and/or recombinant Bacillus construct adjuvant (BCA) containing highly conserved influenza antigens to assess their ability to function as an effective alternative adjuvant in influenza vaccination. In experiment 1, broilers were vaccinated via subcutaneous and/or drinking water application with an inactivated swine influenza in combination with MCA or commercial oil emulsion adjuvant. Subcutaneous or oral prime, followed by oral boost, resulted in the highest improvement in day 21 serum IgG hemagglutination inhibition (HI) titer, compared to the non-vaccinated control group. Experiment 2 tested BCA and MCA adjuvants, mixed with inactivated avian influenza virus in broilers. The group receiving combined subcutaneous and oral prime, followed by an oral boost containing both MCA and BCA adjuvants, exhibited significantly improved tracheal IgA HI titers. Experiment 3 investigated the ability of these adjuvated vaccines to protect against both homologous and heterologous highly pathogenic avian influenza challenge in layers. Groups receiving a subcutaneous prime followed by either subcutaneous or oral boost survived at 100%. Groups receiving only oral vaccine administrations did not exhibit improved survival. MCA and BCA adjuvants were found to improve immune responses in both broiler and layer-type chickens, equivalently matching immunogenicity and protection to the traditional mineral oil emulsion adjuvant. These studies suggest protective mucosal immunity can be achieved with vaccination strategies that incorporate MCA or BCA, but primary parenteral administration remains an important component of the vaccination strategy to achieve protective immunity.



中文翻译:

油性乳剂,甘露糖基化壳聚糖和芽孢杆菌载体佐剂在鸡流感疫苗接种中的比较

在许多国家,针对流感的疫苗接种仍然是主要的控制方法,但是仍然强烈依赖于矿物油乳状液佐剂的自体疫苗,已知该疫苗在注射部位会引起过高的局部炎症反应和损伤。本文介绍的研究研究了甘露糖基化壳聚糖佐剂(MCA)和/或重组芽孢杆菌构建佐剂(BCA)的各种组合)含有高度保守的流感抗原,以评估其在流感疫苗接种中作为有效替代佐剂发挥作用的能力。在实验1中,肉鸡通过皮下注射和/或饮用水与灭活的猪流感疫苗联合MCA或市售油乳剂佐剂进行疫苗接种。与未接种疫苗的对照组相比,皮下或口服初免,然后口服加强剂量,可使第21天的血清IgG血凝凝集抑制(HI)滴度得到最大改善。实验2在肉鸡中测试了BCA和MCA佐剂与灭活禽流感病毒的混合。接受皮下和口服联用的组合,然后同时口服含MCA和BCA佐剂的口服组,其气管IgA HI滴度显着提高。实验3研究了这些混合疫苗在分层中防御同源和异源高致病性禽流感攻击的能力。接受皮下灌注,皮下或口服强化治疗的组存活率为100%。仅接受口服疫苗接种的组没有表现出改善的存活率。发现MCA和BCA佐剂可改善肉鸡和蛋鸡的免疫反应,使免疫原性和保护作用与传统矿物油乳液佐剂相当。这些研究表明,可以通过结合MCA或BCA的疫苗接种策略来实现保护性粘膜免疫,但一次胃肠外给药仍然是实现保护性免疫的疫苗接种策略的重要组成部分。

更新日期:2020-04-30
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