当前位置: X-MOL 学术Gene Expr. Patterns › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Expression of three P4-phospholipid flippases-atp11a, atp11b, and atp11c in zebrafish (Danio rerio).
Gene Expression Patterns ( IF 1.2 ) Pub Date : 2020-04-25 , DOI: 10.1016/j.gep.2020.119115
Alexia Hawkey-Noble 1 , Jurgienne Umali 1 , Gerissa Fowler 1 , Curtis R French 1
Affiliation  

Cellular membrane asymmetry is a hallmark characteristic of all eukaryotic cells. The balance of phospholipid composition within the cytoplasmic inner leaflet and the extracellular outer leaflet of the plasma membrane (PM) maintains cellular function and vitality. The proper exposure of particular phospholipids is necessary to maintain cellular signalling, controlled apoptosis, and vesicle transportation among other roles. Phospholipid asymmetry is coordinated by P4-type phospholipid transferases (flippases or ATPases). ATP11A, ATP11B, and ATP11C belong to class VI of the P4-flippase family (vertebrates) and are responsible for the movement of phosphatidylserine (PS) from the outer leaflet to the inner leaflet of the PM. To date, there is a lack of knowledge of the tissue specific expression of these three flippases on a whole-organism level in a vertebrate system. Here we have determined the spatial-temporal expression profiles of each gene in a zebrafish model using in situ hybridization and performed comparative phylogenetic analyses with other vertebrates. Our data reveals sequence similarity between vertebrate flippases and specific synteny of zebrafish and human chromosomes. Both atp11b and atp11c are maternally expressed in zebrafish, while zygotic expression analysis demonstrates tissue and temporal specificity for all three genes. atp11a is expressed in the neural crest cells as well as in the developing eye and ear, while atp11b is expressed early in the ventricular epithelial lining and later in the ear. atp11c is expressed in the anterior most rhombomeres of the hindbrain, pharyngeal arches, and liver. Our expression data suggests that each of the three flippases are integral for the development of specific tissues, and aberrant function of either could lead to visual, hearing, neural, or liver dysfunction.



中文翻译:

三种P4-磷脂flippases-atp11a,atp11b和atp11c在斑马鱼(Danio rerio)中的表达。

细胞膜不对称性是所有真核细胞的标志性特征。质膜(PM)的细胞质内小叶和细胞外外小叶内磷脂成分的平衡可维持细胞功能和活力。特定磷脂的适当暴露对于维持细胞信号传导,控制细胞凋亡和囊泡运输以及其他作用至关重要。磷脂的不对称性由P4型磷脂转移酶(脂肪酶或ATPase)协调。ATP11AATP11BATP11C属于P4-flippase家族的VI类(脊椎动物),负责磷脂酰丝氨酸(PS)从PM的外部小叶向内部小叶的运动。迄今为止,在脊椎动物系统中,在全生物水平上尚缺乏这三种活菌的组织特异性表达的知识。在这里,我们使用原位杂交确定了斑马鱼模型中每个基因的时空表达谱,并与其他脊椎动物进行了系统进化分析。我们的数据揭示了脊椎动物活菌与斑马鱼和人类染色体的特定同义之间的序列相似性。无论atp11batp11c在斑马鱼中母本表达,而合子表达分析表明这三个基因的组织和时间特异性。atp11a在神经c细胞以及发育中的眼睛和耳朵中表达,而atp11b在心室上皮衬层中早期表达,然后在耳中表达。atp11c表达在后脑,咽弓和肝脏的最前面的菱形。我们的表达数据表明,三个绒毛膜中的每一个对于特定组织的发育都是必不可少的,而任一个的异常功能都可能导致视觉,听觉,神经或肝脏功能障碍。

更新日期:2020-04-25
down
wechat
bug