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Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system.
Molecular Immunology ( IF 3.6 ) Pub Date : 2020-04-30 , DOI: 10.1016/j.molimm.2020.04.017
M M Burucúa 1 , S E Pérez 2 , A C Odeón 3 , E R Cobo 4 , S Quintana 5 , M S Marin 1
Affiliation  

The role of the local innate immune response in the neuropathogenesis of bovine herpesvirus (BoHV) type 1 and 5 remains largely unknown. This study determined the gene transcriptional expression of relevant bovine cathelicidins, TNFα and IFNβ in the nervous system of experimentally-infected cattle during the different stages of BoHV-1 and BoHV-5 infectious cycle. We studied the modulation of bovine myeloid antimicrobial peptide (BMAP) 27 and 28 by alpha-herpesviruses during acute infection of the central nervous system (CNS). However, BMAP28 was the main cathelicidin modulated. BoHV-5 supressed BMAP28 expression mainly in frontal cortex and cervical medulla whereas BoHV-1 slightly induced the expression of cathelicidins in the olfactory and posterior cortex. The differences in the regulation of the innate response are likely related to distinct replication rates of both alpha-herpesviruses in the CNS. During latency and reactivation, BoHV-1 and -5 decreased BMAP28 and BMAP27 expression, accompanied by high levels of TNFα and IFNβ transcripts in the posterior brain region and medulla during BoHV reactivation. In terms of cytokines, a remarkably overexpression of IFNβ was induced by BoHV-5 (133.8-fold). In trigeminal ganglion (TG) both alpha-herpesviruses induced cathelidicins gene expression at all stages of the infection cycle, while only acute BoHV-5 infection increased TNFα (129-fold) mRNA levels. This study suggests that the pronounced downregulation of BMAP28 in BoHV-5-acutely-infected CNS is due to a decreased immune stimulation during viral infection, favouring its establishment in the CNS with a low replication rate until latency. Thus, cathelicidins, together with IFNβ and TNFα, are differentially regulated by BoHV-5 and BoHV-1 infections and this regulation is dependent on the stage of virus infection in the bovine nervous system.

中文翻译:

Cathelicidin牛骨髓抗微生物肽(BMAP)28参与了牛神经系统中针对α-疱疹病毒的炎性反应。

局部先天免疫应答在牛疱疹病毒(BoHV)1型和5型神经病变中的作用仍然未知。这项研究确定了在BoHV-1和BoHV-5感染周期的不同阶段中,实验感染牛的神经系统中相关牛cathelicidins,TNFα和IFNβ的基因转录表达。我们研究了α-疱疹病毒在中枢神经系统(CNS)急性感染过程中对牛骨髓抗微生物肽(BMAP)27和28的调节作用。但是,BMAP28是主要的调制的cathelicidin。BoHV-5抑制了BMAP28的表达,主要在额叶皮质和颈髓中,而BoHV-1轻微诱导了嗅觉和后皮质的cathelicidins表达。先天应答调节的差异可能与两种α-疱疹病毒在中枢神经系统中的不同复制率有关。在潜伏期和重新激活过程中,BoHV-1和-5降低了BMAP28和BMAP27的表达,在BoHV重新激活过程中,在后脑区和延髓中伴有高水平的TNFα和IFNβ转录。就细胞因子而言,BoHV-5诱导了IFNβ的显着过表达(133.8倍)。在三叉神经节(TG)中,两种α-疱疹病毒均在感染周期的所有阶段均诱导了cathelidicins基因表达,而仅急性BoHV-5感染使TNFα(129倍)mRNA水平升高。这项研究表明,在BoHV-5急性感染的中枢神经系统中BMAP28的明显下调是由于病毒感染期间免疫刺激的降低,支持在低延迟的复制率之前在CNS中建立它。因此,cathelicidins与IFNβ和TNFα一起受到BoHV-5和BoHV-1感染的差异调节,这种调节取决于牛神经系统中病毒感染的阶段。
更新日期:2020-04-30
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