Weeds and insect pests are two important biotic stresses resulting in yield loss in rice, and wide compatibility is the essential characteristic of breeding inter-subspecific hybrid rice. This study focused on glyphosate resistance, lepidopteran resistance and wide compatibility as well as identification of molecular and some agronomic characteristics of transgenic male sterile line E1C4008S. The results indicated that glyphosate resistance gene Epsps^# and lepidopteran resistance gene Cry1ca^# were transferred into japonica wide compatibility male sterile line 4008S by Agrobacterium-mediated method, and four independent transformation events named E1C4008S-1, E1C4008S-2, E1C4008S-3 and E1C4008S-4 were obtained, in which E1C4008S-3 and E1C4008S-4 were of single copy insertion. The EPSPS protein contents of E1C4008S-3 and E1C4008S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 300.58 to 1410.69 μg/g at the tillering stage. The glyphosate tolerable concentration (dosage) of E1C4008S-3 and E1C4008S-4 reached at least 16 g/L (54.42 kg/hm²), and the seeds of E1C4008S-4 can germinate normally on the medium containing 1 g/L glyphosate. The CRY1C protein contents of E1C4008S-3 and E1C4008S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 0.62 to 2.43 μg/g at the tillering stage. The larvae mortalities of rice leaf rollers fed on leaves of E1C4008S-3 and E1C4008S-4 for 5 d were 95.35% and 97.77%, respectively, while the average mortalities of silkworms fed with protein extracts from leaves of E1C4008S-3 and E1C4008S-4 reached 94.55% and 83.64%, respectively. The results suggested that wide compatibility and evaluated agronomic traits of E1C4008S-4 were not significantly changed by insertion of the exogenous genes. Overall, a novel male sterile germplasm with glyphosate resistance, lepidopteran resistance and wide compatibility was verified to be developed in rice.

杂草和害虫是导致水稻单产下降的两个重要生物胁迫，广泛的相容性是亚种间杂交水稻育种的基本特征。这项研究的重点是草甘膦抗性，鳞翅目抗性和广泛的相容性，以及转基因雄性不育系E1C4008S的分子和一些农学特性的鉴定。结果表明，草甘膦抗性基因EPSPS ^＃和鳞翅类抗性基因Cry1ca ^＃转移至粳稻由宽的相容性雄性不育系4008S农杆菌介导的方法，获得了四个独立的转化事件，分别为E1C4008S-1，E1C4008S-2，E1C4008S-3和E1C4008S-4，其中E1C4008S-3和E1C4008S-4是单拷贝插入。在不同器官中，E1C4008S-3和E1C4008S-4的EPSPS蛋白质含量以叶>茎>根的降序排列均显着不同，分er期的范围为300.58至1410.69μg/ g。E1C4008S-3和E1C4008S-4的草甘膦耐受浓度（剂量）达到至少16 g / L（54.42 kg / hm ²），而E1C4008S-4的种子可以在含有1 g / L草甘膦的培养基上正常发芽。在不同器官中，E1C4008S-3和E1C4008S-4的CRY1C蛋白含量从叶>茎>根的降序来看均显着不同，分the期的CRY1C蛋白含量在0.62至2.43μg/ g之间。喂食E1C4008S-3和E1C4008S-4叶片5 d的碾米幼虫死亡率分别为95.35％和97.77％，而喂食E1C4008S-3和E1C4008S-4叶片蛋白质提取物的蚕的平均死亡率。分别达到94.55％和83.64％。结果表明，通过插入外源基因，E1C4008S-4的广泛相容性和评估的农艺性状没有明显改变。总体而言，具有草甘膦抗性的新型雄性不育种质