Rice varieties having high Fe concentration in the endospermic region can be used as a good source for Fe deficit population. In this study, 303 Oryza sativa varieties and 1 Oryza rufipogon accession were assessed for spatial Fe accumulation in grains by Prussian blue staining method. Spatial ferritin protein distribution in grains was visualized by immunohistochemistry, and ferritin expression was assessed in selected rice varieties using semi-quantitative reverse transcription PCR. Three popular rice varieties, namely Sarjoo 52, Madhukar and Jalmagna, and the O. rufipogon variety showed Fe in all the regions of grains, and the highest Fe concentration was observed in the embryo region. Some high-yielding varieties like Swarna, Swarna Sub 1, CSR13 and NDRR359 had lower Fe concentration in the embryo region. The highest Fe concentration was detected in O. rufipogon (49.8 μg/g), followed by Sarjoo 52 (26.1 μg/g) and Madhukar (25.7 μg/g). Phytic acid concentration was the minimum in O. rufipogon (5.75 mg/g) followed by Sarjoo 52 (5.83 mg/g). Western blot and semi-quantitative reverse transcription PCR showed higher expression of ferritin gene in O. rufipogon, Sarjoo 52 and Madhukar. In conclusion, O. rufipogon and Sarjoo 52 had higher Fe concentration in the embryo regions as well as endosperm and aleurone layer, whereas the other varieties had lower Fe concentration in the endosperm. Sarjoo 52 could be used as a donor in the rice breeding program for the generation of new varieties with elevated grain Fe concentration.

胚乳区域中高铁浓度的水稻品种可以用作缺铁人群的良好来源。在这项研究中，通过普鲁士蓝染色法评估了303个水稻品种和1个水稻种子的铁积累量。通过免疫组织化学观察谷物中空间铁蛋白蛋白的分布，并使用半定量逆转录PCR评估选定水稻品种中铁蛋白的表达。三种受欢迎的水稻品种，即Sarjoo 52，Madhukar和Jalmagna，以及rufipogon该品种在所有谷粒区域都显示出Fe，而在胚芽区域则观察到最高的Fe浓度。一些高产品种，如Swarna，Swarna Sub 1，CSR13和NDRR359在胚胎区域的铁含量较低。中检测到最高的Fe浓度野生稻（49.8微克/克），随后Sarjoo 52（26.1微克/克）和MADHUKAR（25.7微克/克）。植酸浓度在最小野生稻（5.75毫克/克），随后加入Sarjoo 52（5.83毫克/克）。Western印迹和半定量逆转录PCR结果显示在铁蛋白基因的更高表达野生稻，Sarjoo 52和MADHUKAR。总之，红景天Sarjoo 52在胚区以及胚乳和糊粉层中的铁含量较高，而其他品种在胚乳中的铁含量较低。Sarjoo 52可以作为水稻育种计划的捐助者，以产生铁含量提高的新品种。