In this study, we sequenced the mitochondrial (mt) genome of Agrilus mali (Coleoptera: Buprestidae) using next-generation sequencing, and accordingly annotated 13 protein-coding, 22 tRNA, and 2 rRNA genes and a 1458-bp non-coding region. Comparative analysis indicated that the mt genome of A. mali is relatively conserved, with a typical gene content and order identical to those of other coleopterans. However, the newly sequenced mt genome is characterized by a relatively higher A + T content compared with that of other species within the family Buprestidae. Phylogenetic analysis based on Bayesian inference revealed that the evolutionary relationship among the six infraorders of the suborder Polyphaga is (Scirtiformia + (Elateriformia + ((Scarabaeiformia + Staphyliniformia) + (Bostrichiformia + (Cucujiformia))))). However, the topology indicated that the family Buprestidae is a sister group to other Polyphaga infraorders, excluding Scirtiformia as a monophyly, and thus the monophyly of Elateriformia was not supported. This study not only presents the mt genome of a species in the family Buprestidae and a comparative analysis of jewel beetles but also examines the contribution of mt genomes in elucidating phylogenetic relationships within the suborder Polyphaga of Coleoptera.

中文翻译：

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宝石甲虫（鞘翅目：Buprestidae）的保守线粒体基因组及其对多食亚目的系统发育影响。

在这项研究中，我们使用二代测序对 Agrilus mali (Coleoptera: Buprestidae) 的线粒体 (mt) 基因组进行了测序，并相应地注释了 13 个蛋白质编码基因、22 个 tRNA 和 2 个 rRNA 基因以及一个 1458-bp 的非编码区. 比较分析表明，A. mali 的mt 基因组相对保守，具有典型的基因含量和顺序与其他鞘翅目相同。然而，新测序的 mt 基因组的特点是与 Buprestidae 家族中的其他物种相比，A + T 含量相对较高。基于贝叶斯推断的系统发育分析表明，多食亚目6个亚目之间的进化关系为(Scirtiformia + (Elateriformia + ((Scarabaeiformia + Staphyliniformia) + (Bostrichiformia + (Cucujiformia)))))。然而，拓扑结构表明，Buprestidae 科是其他多食性昆虫纲的姊妹组，不包括作为单系的 Scirtiformia，因此不支持 Elateriformia 的单系。这项研究不仅展示了 Buprestidae 家族中一个物种的 mt 基因组和宝石甲虫的比较分析，而且还检查了 mt 基因组在阐明鞘翅目多食亚目内的系统发育关系方面的贡献。