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Reduction of spermatophore melanization in Litopenaeus vannamei shrimp fed Ulva clathrata during a commercial hatchery production.
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-04-20 , DOI: 10.1016/j.anireprosci.2020.106468
Diana Cristina Corral-Rosales 1 , Denis Ricque-Marie 1 , L Elizabeth Cruz-Suárez 1 , Olivia Arjona-López 2 , Carmen Rodríguez-Jaramillo 2 , Celene Navarro 2 , Elena Palacios 2
Affiliation  

Male broodstock (Litopenaeus vannamei, 36 ± 7 g, n = 600) reproductive performance, spermatophores and reproductive tract melanization, prostaglandin concentrations and biochemical composition were evaluated after including 3.8% Ulva clathrata meal in the diet (dry base) of a commercial hatchery during 45 days. Males fed Ulva had less melanized spermatophores (120 compared with 233, P < 0.01), less bacteria in the ductus deferens (P < 0.01), more sperm cells in testicles (P < 0.05), and increased courtship activity (839 compared with 689, P < 0.01), with no effect on mortality. Ulva-fed males had more arachidonic acid (20:4n-6) in the spermatophores (P < 0.05) but this did not affect the prostaglandin concentrations in response to diet. Males fed Ulva had more carotenoids in the hepatopancreas (0.08 ± 0.02 compared with 0.01 ± 0.01 mg/g, P < 0.05), and phenolic compounds in hepatopancreas (6.1 ± 0.7 compared with 1.8 ± 0.7 mg eq. phloroglucinol/g, P < 0.05) and muscle (0.4 ± 0.3 compared with 0.2 ± 0.1 mg eq. phloroglucinol/g, P < 0.05). Males fed the Ulva also had a lesser carbohydrate content in the hepatopancreas (P < 0.01) and muscle (P < 0.01). In conclusion, supplementing fresh maturation diets with a small dose of dried Ulva allowed for improvement of reproductive performance and to decrease melanization in spermatophores and the male reproductive tract.

中文翻译:

在商业孵化场生产过程中,以南美白对虾喂养的Ulva clathrata精子变黑的减少。

在商业孵化场的日粮(干基)中添加3.8%Ulva clathrata粗粉后,对雄性亲鱼(南美白对虾,36±7 g,n = 600)的繁殖性能,精子和生殖道黑色素,前列腺素的浓度和生化成分进行评估45天 用乌尔瓦(Ulva)喂养的雄性黑色素细胞较少(120比233,P <0.01),输精管中细菌较少(P <0.01),睾丸中的精子细胞更多(P <0.05),求爱活动增加(839比689) ,P <0.01),对死亡率无影响。用Ulva喂养的雄性小鼠的精原细胞中的花生四烯酸含量更高(20:4n-6)(P <0.05),但这并不影响饮食中前列腺素的浓度。饲喂Ulva的雄性肝胰腺中的类胡萝卜素含量更高(0.08±0.02,而0.01±0)。肝胰腺(6.1±0.7与1.8±0.7毫克当量间苯三酚/ g,P <0.05)和肌肉(0.4±0.3与0.2±0.1毫克当量间苯三酚)中的01 mg / g,P <0.05)和酚类化合物/g,P<0.05)。饲喂Ulva的雄性肝胰腺中的碳水化合物含量也较低(P <0.01)和肌肉中的碳水化合物含量(P <0.01)。总之,在新鲜成熟的饮食中添加少量干燥的Ulva可改善生殖性能并减少精原细胞和雄性生殖道的黑色素化。饲喂Ulva的雄性肝胰腺中的碳水化合物含量也较低(P <0.01)和肌肉中的碳水化合物含量(P <0.01)。总之,在新鲜成熟的饮食中添加少量干燥的Ulva可改善生殖性能并减少精原细胞和雄性生殖道的黑色素化。饲喂Ulva的雄性肝胰腺中的碳水化合物含量也较低(P <0.01)和肌肉中的碳水化合物含量(P <0.01)。总之,在新鲜成熟的饮食中添加少量干燥的Ulva可改善生殖性能并减少精原细胞和雄性生殖道的黑色素化。
更新日期:2020-04-20
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