Three different sources of FSH (porcine pituitary, pFSH; recombinant bovine, rbFSH; and recombinant human, rhFSH) were compared during in vitro culture of preantral and early antral follicles of goats for 18 days. Treatments were: base medium supplemented with no FSH (control), 10, 50, or 100 mIU/mL pFSH (pFSH10, pFSH50, and pFSH100, respectively), 100 ng/mL rbFSH (rbFSH), and 50 mIU/mL rhFSH (rhFSH). There were evaluations of follicle morphology, antrum formation, growth rate, estradiol production, oocyte viability and chromatin configuration, and follicle wall relative abundance of mRNA transcript for MMP-9, TIMP-2, CYP17, CYP19A1, FSHR, Insulin-R, and BAX/BCL-2 ratio. Follicle degeneration rates were similar among all treatment groups at the end of culturing. When there were treatments with pFSH, however, there was a lesser (P < 0.05) percentage of intact follicles and estradiol production, and greater (P < 0.05) extrusion rates. Furthermore, with only pFSH10 (antral follicles) and pFSH100 (preantral and antral follicles) treatments, there was a lesser (P < 0.05) follicle growth. For preantral follicles, when there was addition of pFSH10, pFSH100, and rhFSH there was lesser (P < 0.05) oocyte meiotic resumption compared to control and rbFSH treatments. For antral follicles, when there were treatments with rhFSH and pFSH10 there was greater (P = 0.08 – P < 0.05) oocyte maturation. In conclusion, the source of FSH differentially affected gene expression, as indicated by mRNA abundances, and follicular dynamics of preantral and antral follicles in vitro. Addition of FSH during the in vitro culture improved the developmental outcomes only for antral follicles.

在山羊的肛门前和早期肛门卵泡体外培养18天期间，比较了三种不同的FSH来源（猪垂体pFSH；重组牛rbFSH；重组人rhFSH）。处理方法是：补充无FSH（对照），10、50或100 mIU / mL pFSH（分别为pFSH10，pFSH50和pFSH100），100 ng / mL rbFSH（rbFSH）和50 mIU / mL rhFSH（ rhFSH）。对MMP-9，TIMP-2，CYP17，CYP19A1，FSHR，Insulin-R和MMP-9的卵泡形态，胃窦形成，生长速度，雌二醇生成，卵母细胞生存力和染色质构型以及卵泡壁相对丰度进行了评估。BAX / BCL-2比率。在培养结束时，所有治疗组之间的卵泡变性率相似。但是，当使用pFSH进行治疗时，完整卵泡和雌二醇生成的百分比较低（P <0.05），而挤出速率较高（P <0.05）。此外，仅使用pFSH10（前卵泡）和pFSH100（前卵泡和肛门卵泡）治疗，卵泡生长较少（P <0.05）。对于窦前卵泡，与对照和rbFSH处理相比，当添加pFSH10，pFSH100和rhFSH时，卵母细胞减数分裂恢复较少（P <0.05）。对于窦卵泡，当使用rhFSH和pFSH10进行治疗时，则更大（P = 0.08 – P<0.05）卵母细胞成熟。总之，FSH的来源差异影响基因表达，如mRNA丰度以及体外窦前和肛门卵泡的卵泡动力学所表明的。在体外培养过程中添加FSH只能改善肛门卵泡的发育结果。