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Simultaneous measurement of surface and bilayer tension in a microfluidic chip.
Biomicrofluidics ( IF 3.2 ) Pub Date : 2020-04-27 , DOI: 10.1063/1.5137810
Navid Khangholi 1 , Ralf Seemann 1 , Jean-Baptiste Fleury 1
Affiliation  

Freestanding lipid bilayers are one of the most used model systems to mimic biological cell membranes. To form an unsupported bilayer, we employ two aqueous fingers in a microfluidic chip surrounded by an oily phase that contains lipids. Upon pushing two aqueous fingers forward, their interface becomes decorated with a lipid monolayer and eventually zip to form a bilayer when the monolayers have nanoscopic contact with each other. Using this straightforward approach, the quick and easy bilayer formation is facilitated by oil draining into the microfluidic device material consisting of polydimethylsiloxane. However, the oil drainage limits the lifetime of a bilayer to about 1 h. We demonstrate that this drainage can be managed, resulting in superior bilayer stability and an increased lifetime of several hours when using a pressure-controlled system. Applying different pressures to the aqueous fingers in the microfluidic chip, the formed bilayer can even be bent to a desired curvature. Extracting the contact angle and the resulting curvature of the bilayer region, for a given applied pressure difference, both the bilayer tension and the surface tension of each lipid monolayer can be derived from a single experiment using the Young Laplace pressure equation.

中文翻译:

同时测量微流控芯片中的表面和双层张力。

独立的脂质双层是模拟生物细胞膜最常用的模型系统之一。为了形成无支撑的双层,我们在被包含脂质的油相包围的微流控芯片中采用了两个水性手指。向前推动两个水性手指时,它们的界面会被脂质单层修饰,并且当单层相互之间具有纳米级接触时,最终会拉链成双层。使用这种直接的方法,通过将油排入由聚二甲基硅氧烷组成的微流体装置材料中,有助于快速而容易地形成双层。然而,排油将双层的寿命限制为约1小时。我们证明,使用压力控制系统时,可以控制这种排水,从而导致优异的双层稳定性并增加数小时的使用寿命。向微流控芯片中的水指施加不同的压力,所形成的双层甚至可以弯曲到所需的曲率。对于给定的施加压力差,提取双层区域的接触角和所得曲率,可以使用Young Laplace压力方程从单个实验中得出每个脂质单层的双层张力和表面张力。
更新日期:2020-04-27
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