The aim of this study was to determine the effect of benzylpenicillin on the pharmacokinetics of acyclovir in red‐eared slider turtles (Trachemys scripta elegans ). Six clinically healthy red‐eared slider turtles weighing 400 and 580 g were used for the study. Acyclovir (40 mg/kg) and benzylpenicillin (30 mg/kg) were administered intravenously to turtles. In the study, the cross‐pharmacokinetic design (2 × 2) with a 30‐day washout period was performed in two periods. Plasma concentrations of acyclovir were assayed using the high‐performance liquid chromatography with fluorescence detection. Pharmacokinetic parameters were calculated by two‐compartment open pharmacokinetic model. Following the administration of acyclovir alone, elimination half‐life (t _1/2β ), area under the plasma concentration–time curve (AUC), total clearance (Cl_T), and volume of distribution at steady‐state (V _dss) were 20.12 hr, 1,372 hr * µg/mL, 0.03 L hr⁻¹ kg⁻¹, and 0.84 L/kg, respectively. Benzylpenicillin administration increased t _1/2β , AUC, and V _dss while decreased Cl_T of acyclovir. These results showed that benzylpenicillin changed the pharmacokinetics of acyclovir following simultaneous administration in turtles. However, further research is needed to determine molecular mechanism of interaction in turtle.

中文翻译：

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苄青霉素对阿昔洛韦在红耳滑龟（Trachemys scripta elegans）中的静脉药代动力学的影响。

这项研究的目的是确定苄青霉素对红耳滑龟（Austravir elegans）中阿昔洛韦药代动力学的影响。该研究使用了六只临床健康的红耳滑龟，分别为400和580 g。向乌龟静脉注射阿昔洛韦（40 mg / kg）和苄青霉素（30 mg / kg）。在该研究中，分两个阶段进行了具有30天洗脱期的交叉药代动力学设计（2×2）。使用高效液相色谱-荧光检测法测定血浆中无环鸟苷的浓度。通过两室开放式药代动力学模型计算药代动力学参数。单独使用阿昔洛韦后，消除半衰期（t _{1/ 2β} ），血浆浓度-时间曲线（AUC），总清除率（Cl _T）和稳态分布体积（V _dss）下的面积分别为20.12小时，1,372小时* µg / mL，0.03 L小时^-1  kg ^-1和0.84 L / kg。苄青霉素的给药增加了阿昔洛韦的t _{1/ 2β} ，AUC和V _dss，而Cl _T降低。这些结果表明苄青霉素改变了在海龟中同时给药后阿昔洛韦的药代动力学。然而，需要进一步的研究来确定乌龟中相互作用的分子机制。