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Influence of dietary aconitine and nicotine on the gut microbiota of two lepidopteran herbivores.
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2020-04-23 , DOI: 10.1002/arch.21676
Jian-Yong Zeng 1 , De-Dong Wu 1 , Zhong-Bin Shi 1 , Jing Yang 1 , Guo-Cai Zhang 1 , Jie Zhang 2
Affiliation  

The gut microbiota plays an important role in pheromone production, pesticide degradation, vitamin synthesis, and pathogen prevention in the host animal. Therefore, similar to gut morphology and digestive enzyme activity, the gut microbiota may also get altered under plant defensive compound‐induced stress. To test this hypothesis, Dendrolimus superans larvae were fed either aconitine‐ or nicotine‐treated fresh leaves of Larix gmelinii, and Lymantria dispar larvae were fed either aconitine‐ or nicotine‐treated fresh leaves of Salix matsudana. Subsequently, the larvae were sampled 72hr after diet administration and DNA extracted from larval enteric canals were employed for gut microbial 16S ribosomal RNA gene sequencing (338 F and 806 R primers). The sequence analysis revealed that dietary nicotine and aconitine influenced the dominant bacteria in the larval gut and determined their abundance. Moreover, the effect of either aconitine or nicotine on D. superans and L. dispar larvae had a greater dependence on insect species than on secondary plant metabolites. These findings further our understanding of the interaction between herbivores and host plants and the coevolution of plants and insects.

中文翻译:

饮食乌头碱和尼古丁对两种鳞翅类食草动物肠道菌群的影响。

肠道菌群在宿主动物的信息素产生,农药降解,维生素合成和病原体预防中起着重要作用。因此,类似于肠道形态和消化酶活性,在植物防御性化合物诱导的胁迫下,肠道菌群也可能发生改变。为了检验这一假设,落叶松毛虫幼虫喂养任aconitine-或尼古丁处理的新鲜叶子落叶松舞毒蛾幼虫喂任何的aconitine-或尼古丁处理的新鲜叶旱柳。随后,在饮食控制后72小时取样幼虫,将从幼虫肠管提取的DNA用于肠道微生物16S核糖体RNA基因测序(338 F和806 R引物)。序列分析表明,饮食中的尼古丁和乌头碱会影响幼虫肠道中的优势细菌并确定其丰度。此外,乌头碱或尼古丁对D. superansL. dispar幼虫的影响比对次生植物代谢产物的依赖性更大。这些发现进一步加深了我们对食草动物与宿主植物之间相互作用以及植物与昆虫共同进化的理解。
更新日期:2020-04-23
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