In Brazil, the fungus Neopestalotiopsis (= Pestalotiopsis) is known to cause disease in eucalyptus cuttings. However, although it occurs relatively frequently in cutting nurseries, the pathogenic species have yet to be identified. Thus, the aim of the present work was to perform a morphological and phylogenetic characterization to identify the aetiological agent. For this purpose, the isolates were subjected to a multilocus analysis using the two gene regions β‐tubulin (TUB) and the translation elongation factor (TEF). Based on the genomic sequences, two known species and one new species of the pathogen were identified. After confirmation of their pathogenicity, N. australis was confirmed as a new report in eucalyptus. Neopestalotiopsis rosae failed to differ from the control, however, showed internal and external lesion in eucalyptus stem. In addition, in this study, a new species called N. eucalypti was described, causing disease in hybrids eucalyptus. Morphological characterization allowed for the confirmation of the N. australis and N. rosae isolates, primarily based on differences in the size and shape of the conidia. For N. eucalypti, no morphological marker was found that separated it from the other species within the genus. The results confirm the existence of at least three Neopestalotiopsis species as aetiological agents of leaf spot and stem rot in eucalyptus cuttings in Brazil.

中文翻译：

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由新石藻引起的桉树插条茎腐烂。在巴西

在巴西，已知真菌新孢子虫（  Pestalotiopsis）引起桉树插条病害。但是，尽管它在切割苗圃中相对频繁地发生，但尚未确定病原体。因此，本工作的目的是进行形态学和系统发育鉴定以鉴定病因。为此，使用两个基因区域β-微管蛋白（TUB）和翻译延伸因子（TEF）对分离株进行多基因座分析。基于基因组序列，鉴定了病原体的两个已知物种和一个新物种。确认其致病性后，证实澳新猪笼草为桉树的新报道。玫瑰红新陈代谢未能与对照有所不同，但是在桉树茎中表现出内部和外部病变。另外，在这项研究中，描述了一种新的物种，称为桉树，引起杂交桉树的疾病。形态学特征允许主要基于分生孢子的大小和形状的差异来确认澳大利亚猪笼草和玫瑰猪笼草。对于桉树，未发现将其与属内其他物种分离的形态学标记。结果证实在巴西桉树插条中至少存在三种新叶锈菌病种作为叶斑和茎腐病的病原体。