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Diversity analysis of provitamin A maize inbred lines using single nucleotide polymorphism markers
Acta Agriculturae Scandinavica Section B, Soil and Plant Science ( IF 1.6 ) Pub Date : 2020-01-29 , DOI: 10.1080/09064710.2020.1718198
Aleck Kondwakwenda 1 , Julia Sibiya 1 , Assefa B. Amelework 2 , Rebecca Zengeni 1
Affiliation  

The success of a breeding programme can be determined by the level of genetic diversity that exists among breeding materials. This study was carried out to (i) determine the level of genetic diversity that exist among the collection of tropical provitamin A maize inbred lines and (ii) evaluate the genetic structure and divergence of provitamin A maize inbred lines. Forty-six advanced provitamin A inbred lines obtained from the International Maize and Wheat Improvement Centre (CIMMYT) and International Institute of Tropical Agriculture (IITA) were genotyped using 3047 single nucleotide polymorphism (SNP) markers. Genotyping was done following an Illumina Infinium HD Assay Ultra protocol. Eight six percent of the SNPs were polymorphic with the mean polymorphic information content of 0.36. Cluster analysis displayed two distinct clusters. The average pairwise genetic distance among the inbred lines was 0.60. The average gene diversity was 0.359. Variation was partitioned into among individuals (78%), among populations (12%) and within individuals (10%). Overall results suggest the inbred lines are genetically diverse. The key implication of this study is that selection should be done from genetically diverse inbred lines in order to exploit heterosis when developing hybrids.



中文翻译:

利用单核苷酸多态性标记分析维生素原A玉米自交系的多样性

繁殖计划的成功取决于繁殖材料之间存在的遗传多样性水平。进行该研究以(i)确定热带原维生素A玉米自交系的集合中存在的遗传多样性水平,和(ii)评估原维生素A玉米自交系的遗传结构和差异。使用3047单核苷酸多态性(SNP)标记对从国际玉米和小麦改良中心(CIMMYT)和国际热带农业研究所(IITA)获得的46种高级维生素原A自交系进行基因分型。根据Illumina Infinium HD Assay Ultra方案进行基因分型。86%的SNP是多态的,平均多态信息含量为0.36。聚类分析显示了两个不同的聚类。自交系之间的平均成对遗传距离为0.60。平均基因多样性为0.359。变异分为个体间(78%),人群间(12%)和个体内(10%)。总体结果表明,自交系具有遗传多样性。该研究的关键意义在于,应从遗传多样的近交系中进行选择,以便在开发杂种时利用杂种优势。

更新日期:2020-01-29
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