Bioremediation as a low-cost technique can reduce the toxicity of metal ions from the contaminated site including soil and water. In this study, the copper (Cu) and nickel (Ni) bioremediation potential of Microbacterium oxydans strains CM3 and CM7 was investigated under different ranges of environmental factors including pH, temperature, and initial metal concentration by response surface methodology. The coefficients of the regression equation were calculated based on the removal of Cu and Ni by isolated strains. Analysis of variance illustrated a high value of R² for the removal of Cu and Ni by strains CM3 and CM7. The results showed that the percentage of Cu and Ni removal increased with increasing temperature and decreasing pH at low concentration of metal ions. In addition, Cu and Ni were highly removed at pH 5–6 and temperature 36–40°C. Under optimal ranges of the used parameters, the maximum removal percentages of Cu and Ni by strains CM3 and CM7 were 94.15%, 91.11%, 86.32%, and 83.24%, respectively. This study indicates that M. oxydans strains CM3 and CM7 could be used as a powerful alternative to other expensive methods that are used to remove metal ions.

生物修复作为一种低成本技术，可以降低来自受污染场所（包括土壤和水）的金属离子的毒性。在这项研究中，通过响应面方法研究了在不同环境因素（包括pH，温度和初始金属浓度）下，对氧化微细菌菌株CM3和CM7的铜（Cu）和镍（Ni）生物修复潜力。回归方程的系数是基于分离出的菌株对Cu和Ni的去除量来计算的。方差分析表明R ^2的值很高用于通过菌株CM3和CM7去除Cu和Ni。结果表明，在较低的金属离子浓度下，铜和镍的去除率随温度的升高和pH值的降低而增加。此外，在pH 5–6和温度36–40°C时，铜和镍的去除率很高。在最佳使用参数范围内，菌株CM3和CM7对Cu和Ni的最大去除率分别为94.15％，91.11％，86.32％和83.24％。这项研究表明，氧化单胞菌菌株CM3和CM7可以用作其他用于去除金属离子的昂贵方法的有力替代品。