Abstract Eldridge SLC, Wood TM. 2019. Annual variation in microcystin occurrence in Upper Klamath Lake, Oregon, based on high-throughput DNA sequencing, qPCR, and environmental parameters. Lake Reserv Manage. 36:31–44. Cyanobacteria-dominated blooms in Upper Klamath Lake, Oregon, create poor water quality and produce microcystins that may be detrimental to local wildlife and human health. Genetic tools, including high-throughput DNA sequencing and quantitative polymerase chain reaction (qPCR), have been shown to improve the identification and quantification of key groups associated with these blooms over more traditional techniques. We examined the seasonal and interannual variations in nutrient (nitrogen and phosphorus) concentrations between 2013 and 2014 to describe the relations between these factors and the growth dynamics of Aphanizomenon and toxigenic Microcystis as described by DNA sequencing and qPCR. Although total nutrients and chlorophyll a concentrations were similar between years, qPCR results showed the cyanobacterial populations to be 40 times larger in 2014 and indicated a large shift from an Aphanizomenon-dominant, low microcystin-level regime in 2013 to one dominated later in the season by microcystin-producing Microcystis in 2014. In both years, the transition from Aphanizomenon to Microcystis was coincident with a late-season increase in nitrite-plus-nitrate concentrations and in dissolved inorganic nitrogen to dissolved inorganic phosphorus (DIN:DIP) ratios. However, these increases did not explain the large interannual differences in total cyanobacteria abundance. Rather, we hypothesized that year-to-year differences in bioavailable phosphorus, which also manifested as lower total nitrogen to total phosphorus (TN:TP) ratios, were responsible.

中文翻译：

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基于高通量 DNA 测序、qPCR 和环境参数，俄勒冈州上克拉马斯湖微囊藻毒素发生的年度变化

摘要 Eldridge SLC，Wood TM。2019. 基于高通量 DNA 测序、qPCR 和环境参数，俄勒冈州上克拉马斯湖微囊藻毒素发生的年度变化。湖泊保护区管理。36:31-44。俄勒冈州上克拉马斯湖以蓝藻为主的水华导致水质变差并产生可能对当地野生动物和人类健康有害的微囊藻毒素。与更传统的技术相比，包括高通量 DNA 测序和定量聚合酶链反应 (qPCR) 在内的遗传工具已被证明可以改善与这些花朵相关的关键群体的识别和量化。我们检查了 2013 年至 2014 年期间养分（氮和磷）浓度的季节性和年际变化，以描述这些因素与 DNA 测序和 qPCR 所描述的 Aphanizomenon 和产毒微囊藻的生长动态之间的关系。尽管不同年份的总营养物质和叶绿素 a 浓度相似，但 qPCR 结果显示 2014 年蓝藻种群数量增加了 40 倍，并表明从 2013 年的 Aphanizomenon 占主导地位的低微囊藻毒素水平状态大幅转变到本季节后期占主导地位的状态由生产微囊藻毒素的微囊藻于 2014 年发现。在这两年中，从 Aphanizomenon 到微囊藻的转变与亚硝酸盐加硝酸盐浓度和溶解无机氮向溶解无机磷（DIN：DIP) 比率。然而，这些增加并不能解释总蓝藻丰度的巨大年际差异。相反，我们假设生物可利用磷的逐年差异，也表现为较低的总氮与总磷 (TN:TP) 的比率，是负责任的。